MicroRNA profiling and functional validation reveal Poly I:C-responsive miRNAs potentially regulate immune pathways in the giant freshwater prawn, Macrobrachium rosenbergii
Issued Date
2026-01-01
Resource Type
ISSN
10964959
eISSN
18791107
Scopus ID
2-s2.0-105022811668
Pubmed ID
41274401
Journal Title
Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Volume
281
Rights Holder(s)
SCOPUS
Bibliographic Citation
Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology Vol.281 (2026)
Suggested Citation
Thammason R., Pangeson T., Ongvarrasopone C., Wiengnak N., Phetrungnapha A. MicroRNA profiling and functional validation reveal Poly I:C-responsive miRNAs potentially regulate immune pathways in the giant freshwater prawn, Macrobrachium rosenbergii. Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology Vol.281 (2026). doi:10.1016/j.cbpb.2025.111179 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/114789
Title
MicroRNA profiling and functional validation reveal Poly I:C-responsive miRNAs potentially regulate immune pathways in the giant freshwater prawn, Macrobrachium rosenbergii
Corresponding Author(s)
Other Contributor(s)
Abstract
MicroRNAs (miRNAs) are important regulators of gene expression in immune responses. In this study, we examined miRNA expression in M. rosenbergii following stimulation with polyinosinic:polycytidylic acid (Poly I:C), a synthetic analog of viral double-stranded RNA (dsRNA). Small RNA libraries were prepared from control and Poly I:C-injected prawns and sequenced using high-throughput sequencing. A total of 137 differentially expressed miRNAs (DEMs) were identified. Target gene prediction and enrichment analysis showed that these miRNAs are involved in several immune-related pathways, including the ubiquitin–proteasome pathway (UPP), endocytosis, autophagy, and transforming growth factor-β (TGF-β) signaling pathway. Time-course analysis revealed distinct expression patterns of selected miRNAs after Poly I:C injection. Dual-luciferase reporter assays confirmed that mro-miR-7689-3p and mro-miR-9014 directly target nejire ( nej ) and autophagy-related gene 9 ( atg9 ), respectively. These results provide new insights into the role of miRNAs in the antiviral immunity of M. rosenbergii and may support the development of RNA-based strategies for disease control in prawn aquaculture.