The risk of pig and chicken farming for carriage and transmission of Escherichia coli containing extended-spectrum beta-lactamase (ESBL) and mobile colistin resistance (mcr) genes in Thailand
Issued Date
2023-01-01
Resource Type
eISSN
20575858
Scopus ID
2-s2.0-85151042795
Pubmed ID
36951912
Journal Title
Microbial Genomics
Volume
9
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbial Genomics Vol.9 No.3 (2023)
Suggested Citation
Sudatip D., Mostacci N., Tiengrim S., Thamlikitkul V., Chasiri K., Kritiyakan A., Phanprasit W., Thinphovong C., Abdallah R., Baron S.A., Rolain J., Morand S., Oppliger A., Hilty M. The risk of pig and chicken farming for carriage and transmission of Escherichia coli containing extended-spectrum beta-lactamase (ESBL) and mobile colistin resistance (mcr) genes in Thailand. Microbial Genomics Vol.9 No.3 (2023). doi:10.1099/mgen.0.000951 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81959
Title
The risk of pig and chicken farming for carriage and transmission of Escherichia coli containing extended-spectrum beta-lactamase (ESBL) and mobile colistin resistance (mcr) genes in Thailand
Author's Affiliation
Siriraj Hospital
Faculty of Tropical Medicine, Mahidol University
Ubon Ratchathani Rajabhat University
Centre Universitaire de Médecine Générale et Santé Publique, Lausanne
MEPHI - Microbes, Evolution, Phylogénie et Infections
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
University of Bern
Mahidol University
Faculty of Tropical Medicine, Mahidol University
Ubon Ratchathani Rajabhat University
Centre Universitaire de Médecine Générale et Santé Publique, Lausanne
MEPHI - Microbes, Evolution, Phylogénie et Infections
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
University of Bern
Mahidol University
Other Contributor(s)
Abstract
South-East Asian countries report a high prevalence of extended-spectrum cephalosporin-(ESC-) and colistin-resistant Escherichia coli (Col-R-Ec). However, there are still few studies describing the molecular mechanisms and transmission dynamics of ESC-R-Ec and, especially, Col-R-Ec. This study aimed to evaluate the prevalence and transmission dynamics of Ec containing extended spectrum beta-lactamases (ESBL) and mobile colistin resistance (mcr) genes using a 'One Health' design in Thailand. The ESC-R-Ec and Col-R-Ec isolates of human stool samples (69 pig farmers, 155 chicken farmers, and 61 non-farmers), rectal swabs from animals (269 pigs and 318 chickens), and the intestinal contents of 196 rodents were investigated. Resistance mechanisms and transmission dynamics of Ec isolates (n=638) were studied using short and long read sequencing. We found higher rates of ESBL-Ec isolates among pig farmers (n=36; 52.2%) than among chicken farmers (n=58; 37.4 %; P<0.05) and the control group (n=61; 31.1 %; P<0.05). Ec with co-occurring ESBL and mcr genes were found in 17 (6.0 %), 50 (18.6 %) and 15 (4.7 %) samples from humans, pigs and chickens, respectively. We also identified 39 (13.7 %) human samples with non-identical Ec containing ESBL and mcr. We found higher rates of ESBL-Ec, in particular CTX-M-55, isolates among pig farmers than among non-pig farmers (P<0.01). 'Clonal' animal-human transmission of ESBL-Ec and Ec with mcr genes was identified but rare as we overall found a heterogenous population structure of Ec. The Col-R-Ec from human and animal samples often carried mcr-1.1 on conjugative IncX4 plasmids. The latter has been identified in Ec of many different clonal backgrounds.