Sub-chronic oral toxicity of a water-soluble extract of Centella asiatica (Centell-S) in Wistar rats
Issued Date
2024-03-01
Resource Type
ISSN
02786915
eISSN
18736351
Scopus ID
2-s2.0-85184825579
Pubmed ID
38336016
Journal Title
Food and Chemical Toxicology
Volume
185
Rights Holder(s)
SCOPUS
Bibliographic Citation
Food and Chemical Toxicology Vol.185 (2024)
Suggested Citation
Junsai T., Tangpanithandee S., Srimangkornkaew P., Suknuntha K., Vivithanaporn P., Khemawoot P. Sub-chronic oral toxicity of a water-soluble extract of Centella asiatica (Centell-S) in Wistar rats. Food and Chemical Toxicology Vol.185 (2024). doi:10.1016/j.fct.2024.114509 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/97236
Title
Sub-chronic oral toxicity of a water-soluble extract of Centella asiatica (Centell-S) in Wistar rats
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Abstract
Background: Centell-S, a water-soluble extract from Centella asiatica, is predominantly composed of madecassoside and asiaticoside, exceeding 80% w/w. Pursuing its development as an herbal medicinal product, Centell-S underwent sub-chronic toxicity assessment adhering to OECD GLP 408 standards. Methods: In a study involving 100 Wistar rats, varying doses of Centell-S (50, 200, or 800 mg/kg/day) or a vehicle control were administered orally over 90 days. To evaluate Centell-S's safety profile, assessments included clinical observations, health examinations, clinical biochemistry analyses, and detailed anatomical pathology evaluations were conducted. Results: Over the 90 days of treatment, the administration of Centell-S did not lead to any fatalities in the test animals. Clinical observations did not reveal any signs indicative of toxic effects. Notably, an increase in total white blood cell and lymphocyte counts was observed in both sexes, yet these levels returned to normal following a two-week discontinuation period post-treatment. Conclusions: Under the specific conditions of the OECD GLP 408, Repeated Dose 90-day Oral Toxicity Study in Rodents, the no observed adverse effect level (NOAEL) of Centell-S was 800 mg/kg/day. These findings are promising for the continued development of Centell-S as a phytopharmaceutical for clinical applications.