Tamoxifen suppresses expression of prolactin receptor in breast cancer cell lines
2
Issued Date
2024
Copyright Date
2020
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xii, 66 leaves: ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Immunology))--Mahidol University, 2019
Suggested Citation
Kitilak Rochaikun Tamoxifen suppresses expression of prolactin receptor in breast cancer cell lines. Thesis (M.Sc. (Immunology))--Mahidol University, 2019. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/99482
Title
Tamoxifen suppresses expression of prolactin receptor in breast cancer cell lines
Author(s)
Advisor(s)
Abstract
Breast cancer (BCA) is the most common cancer worldwide in women and a highly heterogeneous disease. Human prolactin receptor (PRLR) is a cell surface receptor regularly presented in cells of mammary gland. It is a single pass transmembrane protein that belong to class I cytokine receptor superfamily. It has been reported that the expression in several types of cancer, especially BCA. Tamoxifen is a non-steroidal anti-estrogenic drug which considered for hormone therapy with minimal adverse effects via blocking of estrogen function in BCA cells by competitive binding to estrogen receptor (ER). Previous study has been reported for the decreasing PRLR mRNA expression level after treatment with tamoxifen in BCA patients. However, this finding had not been confirmed the protein expression level by in vitro experiment. This study aimed to investigate the effect of tamoxifen in PRLR expression at protein level on BCA cell lines. The expression of PRLR in BCA cell lines including T47D, MCF-7, MDA-MB-231, HCC70, BCA55-121, PC-B-120CA and PC-B-142CA were observed by immunofluorescence analysis (IFA) and western blot analysis and further observed the localization by confocal microscopy analysis. The treatment of tamoxifen on BCA cells and detection of PRLR protein expression was performed. Only T47D, MCF-7, HCC70 and PC-B-142CA cells showed PRLR expression by both IFA and western blot. Western blot results also showed different isoforms of PRLR expression on each BCA cell line. Confocal microscopy analysis determined high membrane expression in T47D cells while only cytoplasmic expression in MCF-7, HCC70 and PC-B-142CA cells. Treatment with tamoxifen showed down-regulation of PRLR in T47D and MCF-7 cells while HCC70 did not change. In conclusion, tamoxifen showed the effect to down-regulate PRLR protein expression in BCA cell lines, confirmed previous finding in BCA patients. This may indicate the other consideration of using tamoxifen in therapeutic approach of BCA patients.
Description
Immunology (Mahidol University 2020)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Medicine Siriraj Hospital
Degree Discipline
Immunology
Degree Grantor(s)
Mahidol University