Glucose-6-phosphate dehydrogenase is dispensable for human erythroid cell differentiation in vitro
2
Issued Date
2023-05-01
Resource Type
ISSN
0301472X
eISSN
18732399
Scopus ID
2-s2.0-85149894302
Pubmed ID
36801436
Journal Title
Experimental Hematology
Volume
121
Start Page
18
End Page
29.e2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Experimental Hematology Vol.121 (2023) , 18-29.e2
Suggested Citation
Boonpeng K., Ketprasit N., Palasuwan A., Kulkeaw K., Palasuwan D. Glucose-6-phosphate dehydrogenase is dispensable for human erythroid cell differentiation in vitro. Experimental Hematology Vol.121 (2023) , 18-29.e2. 29.e2. doi:10.1016/j.exphem.2023.02.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81597
Title
Glucose-6-phosphate dehydrogenase is dispensable for human erythroid cell differentiation in vitro
Author's Affiliation
Other Contributor(s)
Abstract
Glucose-6-phosphate dehydrogenase (G6PD) deficiency impairs cellular processes under oxidative stress. Individuals with severe G6PD deficiency still produce sufficient numbers of erythrocytes. Nevertheless, the G6PD independence of erythropoiesis remains questionable. This study elucidates the effects of G6PD deficiency on the generation of human erythrocytes. Peripheral blood-derived CD34-positive hematopoietic stem and progenitor cells (HSPCs) of human subjects with normal, moderate, and severe G6PD activities were cultured in two distinct phases: erythroid commitment and terminal differentiation. Regardless of G6PD deficiency, HSPCs were able to proliferate and differentiate into mature erythrocytes. There was no impairment in erythroid enucleation among the subjects with G6PD deficiency. To our knowledge, this study is the first report of effective erythropoiesis independent of G6PD deficiency. The evidence firmly indicates that the population with the G6PD variant could produce erythrocytes to an extent similar to that in healthy individuals.