The Effect of Uvaria rufa Fruit Extract for Antioxidant and Anti-Melanogenic Activities and Phytochemical Profiling Using LC–MS Analysis
4
Issued Date
2026-01-01
Resource Type
ISSN
1096620X
eISSN
15577600
Scopus ID
2-s2.0-105030507325
Pubmed ID
41144290
Journal Title
Journal of Medicinal Food
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Medicinal Food (2026)
Suggested Citation
Tangpudtasiri P., Wiriyakun N., Niwaspragrit C., Amornlerdpison D., Namchaiw P., Ratanachamnong P., Jaisin Y. The Effect of Uvaria rufa Fruit Extract for Antioxidant and Anti-Melanogenic Activities and Phytochemical Profiling Using LC–MS Analysis. Journal of Medicinal Food (2026). doi:10.1177/1096620X251388848 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/115408
Title
The Effect of Uvaria rufa Fruit Extract for Antioxidant and Anti-Melanogenic Activities and Phytochemical Profiling Using LC–MS Analysis
Author's Affiliation
King Mongkut's University of Technology Thonburi
Faculty of Science, Mahidol University
Thailand National Science and Technology Development Agency
Srinakharinwirot University
Maejo University
Thailand Institute of Scientific and Technological Research (TISTR)
Faculty of Medicine, Srinakharinwirot University
Faculty of Science, Mahidol University
Thailand National Science and Technology Development Agency
Srinakharinwirot University
Maejo University
Thailand Institute of Scientific and Technological Research (TISTR)
Faculty of Medicine, Srinakharinwirot University
Corresponding Author(s)
Other Contributor(s)
Abstract
Uvaria rufa (U. rufa) Blume is commonly found in Southern and Eastern Asia, including Thailand. This plant is commonly known as “Carabao teats” in English and “Pee Puan” in Thai. Even though hyperpigmented skin is generally a harmless condition, it is often a cosmetic concern. Ultraviolet exposure seems to be the primary contributing factor. In this study, we investigated the bioactive constituents of the ethanol extract of U. rufa fruits and evaluated their anti-melanogenic effects and tyrosinase inhibition in B16F10 mouse melanocyte cells (B16F10 melanoma cells) induced by α-melanocyte-stimulating hormone (α-MSH). This extract included phenolic and flavonoid components, as well as secondary metabolites demonstrated using LC–MS analysis, including betaine, hydroquinone, kojic acid, nicotinamide, quercetin, cysteamine, neoagarobiose, gallic acid, and cinnamic acid. Based on its antioxidant constituents and preliminary antioxidant screening, the extract possessed free radical scavenging capability by effectively scavenging DPPH and nitric oxide (NO) radicals with an IC<inf>50</inf> of 7.05 and 16.56 mg/mL, respectively. The extract inhibited melanin production by 123.2 ± 3.3% and tyrosinase activity by 134 ± 4.23% in α-MSH-stimulated B16F10 cells, suggesting that the observed anti-melanogenic capability arises from the combined action of several bioactive substances. Taken together, this present study demonstrates that U. rufa fruits have potential as a natural anti-melanogenic remedy and supports its further development for applications in the treatment of hyperpigmentation.