Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies
Issued Date
2024-02-01
Resource Type
eISSN
27740226
Scopus ID
2-s2.0-85184402636
Journal Title
Trends in Sciences
Volume
21
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Trends in Sciences Vol.21 No.2 (2024)
Suggested Citation
Vivithanaporn P., Siangcham T., Tanawoot V., Settacomkul R., Pranweerapaiboon K., Meemon K., Niamnont N., Tamtin M., Sobhon P., Sangpairoj K. Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies. Trends in Sciences Vol.21 No.2 (2024). doi:10.48048/tis.2024.7157 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97207
Title
Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies
Corresponding Author(s)
Other Contributor(s)
Abstract
Glioblastoma (GBM) considered as aggressive brain cancer with high mortality rate in patients even after surgical resection. Resistant to chemotherapy is the major problem in GBM therapy. Discovery of novel bioactive compounds from algae is being investigated as alternative sources for potential treatment as well as prevention in glioblastoma. This study revealed the effects of marine red algae extract from hexane solvent fraction of Halymenia durvillei (HDHE) on proliferation and cell death in A172 human GBM cells. HDHE decreased proliferation and promoted cell cycle arrest at G2/M phase. HDHE induced apoptotic cell death in A172 cells through mitochondrial membrane dysfunction, the decrease of anti-apoptotic Bcl-2 protein expression, and activation of caspase 3/7. Moreover, HDHE increased intracellular reactive oxygen species (ROS) production and accumulation of LC3-II, an autophagic marker. The docked conformation of palmitic acid, a major component of HDHE, showed a high affinity binding to TP53 and Beclin-1 as cell death-related target molecules. This research conclusively demonstrated that HDHE might serve as a potent anticancer agent against glioblastoma by promoting apoptotic and autophagic cell death in A172 human GBM cells.