Analysis of crude and excretory-secretory antigens of Dirofilaria immitis adult worms against antibody of brugian filariasis by elisa and immunoblot
Issued Date
2007
Copyright Date
2007
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xxiii, 128 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Tropical Medicine))--Mahidol University, 2007
Suggested Citation
Panupong Sahaisook Analysis of crude and excretory-secretory antigens of Dirofilaria immitis adult worms against antibody of brugian filariasis by elisa and immunoblot. Thesis (M.Sc. (Tropical Medicine))--Mahidol University, 2007. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/96712
Title
Analysis of crude and excretory-secretory antigens of Dirofilaria immitis adult worms against antibody of brugian filariasis by elisa and immunoblot
Alternative Title(s)
การวิเคราะห์แอนติเจนชนิดสกัดอย่างหยาบและชนิดสารคัดหลั่งจากพยาธิหัวใจสุนัขระยะตัวเต็มวัยต่อแอนติบอดีต่อโรคเท้าช้างชนิดบรูเกียนด้วยวิธี elisa และ immunoblot
Author(s)
Abstract
Serodiagnosis of Brugian filariasis is not available in Thailand and a good test has not been demonstrated in the literature. Therefore, this study evaluated excretorysecretory antigens, either non-delipidized (NDLP) or delipidized (DLP) crude extracts from Dirofilaria immitis fresh and post-RPMI 1640 cultured adult worms, from which NDLP-fresh male and ether-DLP-fresh female crude extracts were subsequently selected by immunoblot test using pooled Brugian and negative sera to discriminate the unsatisfied extracts. IgG-ELISA analyzed male ES antigen (0.5 ug/ml), 1:800- diluted sera of Brugian filariasis (36 cases), other parasitic infections (181) and negative controls (54), and 1:8,000 dilution of IgG-conjugate. The test gave 100% sensitivity and 96.17% specificity at cut-off value 0.587. Only 8 cases of six parasitic infections (4.42%) and 1 negative control (1.85%) were false-positive. Under the above conditions, ES female antigen produced 100% sensitivity and 81.12% specificity at cut-off value 0.616. False positives were found in 37 cases of twenty parasitic infections (20.11%) and 8 negative controls (14.81%). For NDLP-fresh male antigen (2 ug/ml), serum dilution (1:1,600), and IgG-conjugate dilution (1:8,000), gave 88.89% sensitivity and 81.28% specificity, at cut-off value 0.233. The DLPfresh female antigen used the same conditions as the male crude antigen, except for IgG-conjugate at 1:16,000, resulting in 94.44% sensitivity and 76.17% specificity at cut-off value 0.164. Twenty parasitic infections showed false positives, 24.31% (44/181) and 30.94% (56/181) for crude male and female antigens, respectively. By immunoblot, only fresh male crude antigen showed an interesting glycoprotein band of 20.62 kDa, but it was inconsistent with Brugian filariasis sera and also cross-reacted with Bancroftian filariasis (5/5), gnathostomiasis (1/3), and angiostrongyliasis (1/3). It was concluded that ES male antigen can probably be used in the serodiagnosis of Brugian filariasis by IgG-ELISA, and male crude antigen may be used in detecting lymphatic filariasis by IgG-immunoblot. However, a purified antigen is needed for further study.
Description
Tropical Medicine (Mahidol University 2007)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Tropical Medicine
Degree Discipline
Tropical Medicine
Degree Grantor(s)
Mahidol University