Curcuma sp. “Khamin Oi” extracts inhibit human coronavirus OC43 replication in HCT-8 colorectal cell line
Issued Date
2024-12-15
Resource Type
eISSN
24058440
Scopus ID
2-s2.0-85209626050
Journal Title
Heliyon
Volume
10
Issue
23
Rights Holder(s)
SCOPUS
Bibliographic Citation
Heliyon Vol.10 No.23 (2024)
Suggested Citation
Pichetpongtorn P., Ruangdachsuwan S., Churod T., Komaikul J., Masrinoul P., Yusakul G., Kitisripanya T. Curcuma sp. “Khamin Oi” extracts inhibit human coronavirus OC43 replication in HCT-8 colorectal cell line. Heliyon Vol.10 No.23 (2024). doi:10.1016/j.heliyon.2024.e40569 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/102188
Title
Curcuma sp. “Khamin Oi” extracts inhibit human coronavirus OC43 replication in HCT-8 colorectal cell line
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Abstract
Plants belonging to the genus Curcuma have shown promise in exerting antiviral activity. In this study, Curcuma sp. “Khamin Oi” (CKO), a traditional Thai medicinal herb listed in Thai herbal pharmacopoeia, was subjected to extraction using a variety of solvents. Consequently, LC-MS/MS and GC-MS/MS analyses were conducted to assess the phytochemical profiles of all the crude extracts and identify the compounds potentially responsible for their antiviral effects. The antiviral effects of these extracts against the human coronavirus OC43 (HCoV-OC43) were also investigated. An in-cell ELISA was used to investigate the anti-HCoV-OC43 replication activity of the crude rhizome extracts of CKO. Among the extracts, the crude hexane extract of CKO exhibited the lowest IC50 (3.62 μg/mL), with a high selectivity index (SI) of 31.90, despite having the lowest curcuminoid content. While the methanolic extract of Curcuma longa L., known for its high curcuminoid content among Curcuma species, showed an IC50 of 14.83 μg/mL with an SI of 2.80. Further in vitro and in vivo investigations of CKO crude extract are necessary to understand its potential anti-HCoV-OC43 properties.