The Contribution of Polyaniline and Gold Nanoparticles for Enhancing Signals of Streptavidin Based Immunosensor
9
Issued Date
2022-01-01
Resource Type
ISSN
19386737
eISSN
19385862
Scopus ID
2-s2.0-85130563151
Journal Title
ECS Transactions
Volume
107
Issue
1
Start Page
827
End Page
836
Rights Holder(s)
SCOPUS
Bibliographic Citation
ECS Transactions Vol.107 No.1 (2022) , 827-836
Suggested Citation
Samae H., Thamphiwatana S.D., Phairatana T. The Contribution of Polyaniline and Gold Nanoparticles for Enhancing Signals of Streptavidin Based Immunosensor. ECS Transactions Vol.107 No.1 (2022) , 827-836. 836. doi:10.1149/10701.0827ecst Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84640
Title
The Contribution of Polyaniline and Gold Nanoparticles for Enhancing Signals of Streptavidin Based Immunosensor
Author(s)
Other Contributor(s)
Abstract
The presence of streptavidin has been found to serve as an electrically insulating layer. This could reduce the initial current response of an electrochemical immunosensor, reducing its performance for detecting the analyte of interest. The work aimed to investigate the current response of the streptavidin based electrochemical immunosensor at polyaniline and gold nanoparticle modified screen-printed electrodes and compare to without gold nanoparticle addition. The result of the streptavidin modified gold nanoparticles - polyaniline sensor exhibited a three-times higher current response than that of the streptavidin modified polyaniline sensor (without gold nanoparticle). Additionally, antibody immobilization (EpCAM as a model) on the modified electrode showed high performances for use further in antigen target detection. The high conductivity of the developed immunosensor could become a promising candidate for immunosensor development with high sensitivity in the future.