Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
Issued Date
2022-12-20
Resource Type
ISSN
00345288
eISSN
15322661
Scopus ID
2-s2.0-85137616172
Pubmed ID
36108550
Journal Title
Research in Veterinary Science
Volume
152
Start Page
372
End Page
386
Rights Holder(s)
SCOPUS
Bibliographic Citation
Research in Veterinary Science Vol.152 (2022) , 372-386
Suggested Citation
Junsiri W., Watthanadirek A., Poolsawat N., Minsakorn S., Srionrod N., Nooroong P., Sangchuai S., Chawengkirttikul R., Glab-ampai K., Anuracpreeda P. Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2. Research in Veterinary Science Vol.152 (2022) , 372-386. 386. doi:10.1016/j.rvsc.2022.08.019 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/87066
Title
Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
Other Contributor(s)
Abstract
A. marginale's major surface protein 2 (MSP2) is an immunodominant protein that is encoded by a multigene family. Phylogenetic analysis revealed that the msp2 sequence Thailand strain was clustered in third clade, with similarity values between 90.4 and 100%. The haplotype diversity showed 10 haplotypes of the msp2 genes. The entropy analysis of the nucleic and amino sequences revealed 289 and 117 high entropy peaks, respectively. Interestingly, one predicted allele belonging to MHC-II represented the hypervariable region (HVR) of MSP2. A. marginale's recombinant MSP2 (rAmMSP2), which has a molecular weight of 42 kDa, was examined in SDS-PAGE. Antigenicity of rAmMSP2 (42 kDa) and AmMSP2 (36 kDa) showed the conserved epitopes. The distribution of AmMSP2 on infected erythrocytes' membrane and outside was demonstrated by immunofluorescence detection. Therefore, the rMSP2 could be utilized in the establishment of immunodiagnostic tools and vaccine approaches for the monitoring of anaplasmosis.