cDNA expression library and immunoscreening of thai paragonimus heterotremus

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dc.contributor.authorKanokkarn Pothongen_US
dc.contributor.authorกนกกาญจน์ โพธิ์ทองen_US
dc.contributor.authorParon Dekumyoyen_US
dc.contributor.authorพารณ ดีคำย้อยen_US
dc.contributor.authorThareerat Kalambahetien_US
dc.contributor.authorธารีรัตน์ กะลัมพะเหติen_US
dc.contributor.authorDorn Watthanakulpanichen_US
dc.contributor.authorดร วัฒนกุลพานิชย์en_US
dc.contributor.authorChalit Komalmisraen_US
dc.contributor.authorชลิต โกมลมิศร์en_US
dc.contributor.authorTimothy P Yoshinoen_US
dc.contributor.otherMahidol University. Faculty of Tropical Medicine. Department of Helminthologyen_US
dc.contributor.otherMahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunologyen_US
dc.date.accessioned2015-08-17T08:13:07Z
dc.date.accessioned2021-08-17T06:53:45Z
dc.date.available2015-08-17T08:13:07Z
dc.date.available2021-08-17T06:53:45Z
dc.date.created2015-08-17
dc.date.issued2014
dc.descriptionJoint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 278.en
dc.description.abstractA cDNA expression library of Paragonimus heterotremus, the main causative agent of human paragonimiasis in Thailand, was performed to produce recombinant antigens. To identify the antigenic clones, immunized sera were tested using immunoscreening. Ten positive clones were selected, and the inserted genes partially sequenced. The sequencing data were analyzed using BLASTp searches. Six clones were found to contain a gene insert that encoded a protein similar to that found in helminthic worms (Clonorchis sinensis, Schistosoma mansoni, Schistosoma japonicum, Echinococcus multilocularis, Brugia malayi), two clones similar to Toxoplasma gondii, while two more clones were shown to encode proteins similar to that of Plasmodium chabaudi chabaudiand Microcystis spp. (uncultured). The clones will be further studied in term of protein expression through recombinant antigen evaluation using human paragonimiasis and other parasitic infection sera.en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/63173
dc.language.isoengen_US
dc.rightsMahidol Universityen_US
dc.subjectParagonimus heterotemusen_US
dc.subjectcDNAen_US
dc.titlecDNA expression library and immunoscreening of thai paragonimus heterotremusen_US
dc.typeProceeding Posteren_US
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