Modulation of sepsis by Lacticaseibacillus rhamnosus and the potential role of short-chain fatty acid levels in feces and blood
Issued Date
2026-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-105028523218
Pubmed ID
41444762
Journal Title
Scientific Reports
Volume
16
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.16 No.1 (2026)
Suggested Citation
Chancharoenthana W., Kamolratanakul S., Pinitchun C., Vorapreechapanich A., Wannigama D.L., Somboonna N., Cheibchalard T., Settachaimongkon S., Schultz M.J., Leelahavanichkul A. Modulation of sepsis by Lacticaseibacillus rhamnosus and the potential role of short-chain fatty acid levels in feces and blood. Scientific Reports Vol.16 No.1 (2026). doi:10.1038/s41598-025-33032-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/114698
Title
Modulation of sepsis by Lacticaseibacillus rhamnosus and the potential role of short-chain fatty acid levels in feces and blood
Author's Affiliation
Universiteit van Amsterdam
The University of Sheffield
Chulalongkorn University
Nuffield Department of Medicine
Faculty of Tropical Medicine, Mahidol University
Faculty of Medicine, Chulalongkorn University
Mahidol Oxford Tropical Medicine Research Unit
Yamagata Prefectural Central Hospital
Yamagata University Hospital
Yamagata Prefectural University of Health Sciences
The University of Sheffield
Chulalongkorn University
Nuffield Department of Medicine
Faculty of Tropical Medicine, Mahidol University
Faculty of Medicine, Chulalongkorn University
Mahidol Oxford Tropical Medicine Research Unit
Yamagata Prefectural Central Hospital
Yamagata University Hospital
Yamagata Prefectural University of Health Sciences
Corresponding Author(s)
Other Contributor(s)
Abstract
The efficacy of probiotics for sepsis attenuation might be associated with the alteration of short-chain fatty acids (SCFAs). We investigated the impact of probiotics with the different production of SCFAs in vitro, including Lacticaseibacillus rhamnosus strains fa1 and fg2 in mice with cecal ligation and puncture. Administration of either fa1 or fg2 probiotics, but not the heat-killed probiotics, prior to surgery effectively reduced sepsis severity. Metabolome analysis revealed elevated levels of acetate and 3-hydroxybutyrate in blood, whereas butyrate and propionate levels were diminished in the feces of sepsis mice compared to sham controls. Both probiotics similarly attenuated sepsis-induced gut dysbiosis, as indicated by the normalized Firmicutes and reduced Proteobacteria (fecal microbiome analysis), with the similar levels of fecal SCFAs. In parallel, the administration of butyrate, but not acetate, partly attenuated sepsis severity (gut permeability and serum TNF-α). Conditioned media from both probiotic strains or butyrate demonstrated a protective effect against enterocyte injury following activation by Klebsiella pneumoniae lysate, irrespective of their SCFAs production. To support the possible use of SCFAs in sepsis, the lower serum SCFAs in patients with sepsis compared to healthy controls was demonstrated. In conclusion, both fa1 and fg2 attenuated sepsis severity, partly through the increased levels of SCFAs. These findings endorse the potential of probiotics in preventing sepsis and the use of SCFAs for sepsis disease monitoring.