Supplementation of Bacillus velezensis S141 in feed as a probiotic enhances growth performance, pathogenic tolerances, and immune system in shrimp

dc.contributor.authorSeabkongseng T.
dc.contributor.authorLimkul S.
dc.contributor.authorSriphuttha C.
dc.contributor.authorPhiwthong T.
dc.contributor.authorAunkam P.
dc.contributor.authorSuwannathit R.
dc.contributor.authorJaree P.
dc.contributor.authorSomboonwiwat K.
dc.contributor.authorTittabutr P.
dc.contributor.authorTeaumroong N.
dc.contributor.authorBoonchuen P.
dc.contributor.correspondenceSeabkongseng T.
dc.contributor.otherMahidol University
dc.date.accessioned2025-04-02T18:09:57Z
dc.date.available2025-04-02T18:09:57Z
dc.date.issued2025-06-30
dc.description.abstractIn Litopenaeus vannamei aquaculture, infectious diseases pose significant challenges, leading to the exploration of alternative strategies for pathogen combat. This study examines the efficacy of Bacillus velezensis S141, supplemented in feed at various levels 102, 104, and 106 CFU/g, on immune responses, growth performance, and disease tolerances against the White Spot Syndrome Virus (WSSV), Vibrio parahaemolyticus (VPAHPND), and Enterocytozoon hepatopenaei (EHP). The results indicated significant improvements in the weight gain (WG), average daily gain (ADG), and specific growth rate (SGR) of shrimp supplemented with B. velezensis S141 compared to the control group. Moreover, shrimp supplemented with B. velezensis S141 had significantly higher survival rates during post-WSSV infection. Co-injection with B. velezensis S141 secretion and WSSV significantly improved survival rates and reduced WSSV copy numbers in the gill, indicating enhanced tolerance to the WSSV infection. Administering B. velezensis S141 reduced cumulative mortality during the VPAHPND challenge and EHP copy number compared to the control group. Furthermore, the gene expression analyses of immune-related genes in the Toll/IMD pathway (Toll, IMD, LYZ1, LYZ-C, PEN4, ALF1, Relish, NF-kappa, RPX, and DOME), JAK/STAT pathway (STAT and GILT), Vago pathway (Vago4, Vago5), CathC, and α2M showed significant upregulation in the group administered feed containing 106 CFU/g of B. velezensis S141 compared to the control group in the gills. This study emphasizes the potential of B. velezensis S141 as a probiotic supplement in enhancing immune responses and disease tolerances against WSSV, EHP, and VPAHPND in L. vannamei, providing promising prospects for sustainable shrimp aquaculture practices.
dc.identifier.citationAquaculture Vol.604 (2025)
dc.identifier.doi10.1016/j.aquaculture.2025.742448
dc.identifier.issn00448486
dc.identifier.scopus2-s2.0-105000610204
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/108599
dc.rights.holderSCOPUS
dc.subjectAgricultural and Biological Sciences
dc.titleSupplementation of Bacillus velezensis S141 in feed as a probiotic enhances growth performance, pathogenic tolerances, and immune system in shrimp
dc.typeArticle
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105000610204&origin=inward
oaire.citation.titleAquaculture
oaire.citation.volume604
oairecerif.author.affiliationChulalongkorn University
oairecerif.author.affiliationSuranaree University of Technology
oairecerif.author.affiliationInstitute of Molecular Biosciences, Mahidol University

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