Pathogenic Leptospira Detection in Environmental Contaminant Water Sources by Highly Performance Antibody Absorption Polystyrene Agglutinating Particles
Issued Date
2024-01-01
Resource Type
ISSN
09340866
eISSN
15214117
Scopus ID
2-s2.0-85200461633
Journal Title
Particle and Particle Systems Characterization
Rights Holder(s)
SCOPUS
Bibliographic Citation
Particle and Particle Systems Characterization (2024)
Suggested Citation
Suwannin P., Polpanich D., Lebaz N., Saimuang K., Jindakaew J., Tangboriboonrat P., Jangpatarapongsa K., Elaissari A. Pathogenic Leptospira Detection in Environmental Contaminant Water Sources by Highly Performance Antibody Absorption Polystyrene Agglutinating Particles. Particle and Particle Systems Characterization (2024). doi:10.1002/ppsc.202400023 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/100473
Title
Pathogenic Leptospira Detection in Environmental Contaminant Water Sources by Highly Performance Antibody Absorption Polystyrene Agglutinating Particles
Corresponding Author(s)
Other Contributor(s)
Abstract
Leptospirosis is a re-emerging bacterial zoonotic disease that affects both humans and animals, with a significantly higher incidence in tropical and sub-tropical regions. Disease control, epidemiology, and surveillance rely on a One Health approach, as accurate detection can be applied to humans, animals, and the environment. This study represents the first attempt to develop a method for detecting the pathogenic Leptospira santarosai serovar Shermani based on the latex agglutination reaction. The serological activity of the antibody is examined to achieve a high titer of antibody before adsorption onto polystyrene particles. Using a pH medium of 6.8–7.8, total antibody adsorption of up to 3 mg m−2 is achieved. Particle agglutination is observed after incubating the antibody-adsorbed PS with leptospiral culture for 4 min, revealing a detection limit of 1.7 × 102 leptospires mL−1. Interestingly, the detection limit increased by 1000 times when observing agglutination using spectrophotometer. The test exhibits high specificity with Shermani and shows negligible cross-agglutination with non-pathogenic Leptospira and water-borne bacteria. Agglutination testing in collected water samples from natural sources demonstrates a good correlation with culture technique. This simple and rapid leptospires agglutination detection method can be applied as a screening test in environmental, human, and animal specimens.