Episomal vector-based generation of human induced pluripotent stem cell line MUSIi020-A from peripheral blood T-cells

Issued Date

2022-10-01

Resource Type

Article

ISSN

18735061

eISSN

18767753

DOI

10.1016/j.scr.2022.102929

Scopus ID

2-s2.0-85139076055

Pubmed ID

36183677

Journal Title

Stem Cell Research

Volume

64

Rights Holder(s)

SCOPUS

Bibliographic Citation

Stem Cell Research Vol.64 (2022)

Suggested Citation

Pratumkaew P., Luanpitpong S., Klaihmon P., Lorthongpanich C., Laowtammathron C., Meesa S., Suksomboon W., Issaragrisil S. Episomal vector-based generation of human induced pluripotent stem cell line MUSIi020-A from peripheral blood T-cells. Stem Cell Research Vol.64 (2022). doi:10.1016/j.scr.2022.102929 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83597

Title

Episomal vector-based generation of human induced pluripotent stem cell line MUSIi020-A from peripheral blood T-cells

Author(s)

Pratumkaew P.
 Luanpitpong S.
 Klaihmon P.
 Lorthongpanich C.
 Laowtammathron C.
 Meesa S.
 Suksomboon W.
 Issaragrisil S.

Author's Affiliation

Siriraj Hospital

Other Contributor(s)

Mahidol University

Abstract

Human induced pluripotent stem cell (iPSC) line MUSIi020-A was generated from T cells isolated from peripheral blood of a healthy 37-year-old female and reprogrammed using episomal plasmid vectors. The established transgene-free MUSIi020-A, which retained a normal karyotype, displayed pluripotency as characterized by expression of pluripotency markers and by in vitro spontaneous differentiation toward three embryonic germ layers. This cell line may represent a valuable tool for studying T cell development and a potential cell source for cancer immunotherapy.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/83597

Collections

Scopus 2022