Low phosphate mitigates cadmium-induced oxidative stress in Saccharomyces cerevisiae by enhancing endogenous antioxidant defence system
Issued Date
2022-02-01
Resource Type
ISSN
14622912
eISSN
14622920
Scopus ID
2-s2.0-85121455364
Pubmed ID
34927334
Journal Title
Environmental Microbiology
Volume
24
Issue
2
Start Page
707
End Page
720
Rights Holder(s)
SCOPUS
Bibliographic Citation
Environmental Microbiology Vol.24 No.2 (2022) , 707-720
Suggested Citation
Kerdsomboon K., Techo T., Limcharoensuk T., Tatip S., Auesukaree C. Low phosphate mitigates cadmium-induced oxidative stress in Saccharomyces cerevisiae by enhancing endogenous antioxidant defence system. Environmental Microbiology Vol.24 No.2 (2022) , 707-720. 720. doi:10.1111/1462-2920.15875 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83334
Title
Low phosphate mitigates cadmium-induced oxidative stress in Saccharomyces cerevisiae by enhancing endogenous antioxidant defence system
Other Contributor(s)
Abstract
Cadmium is a highly toxic heavy metal that causes many harmful effects on human health and ecosystems. Metal chelation-based techniques have become a common approach for the treatment of metal poisoning and also for the remediation of metal contamination. Phosphate, an essential nutrient required for key cellular functions, has been supposed to be effective in reducing cadmium bioavailability, possibly through its chelating potential. In this study, we explored the effects of phosphate on cadmium toxicity and cellular response to cadmium stress in the eukaryotic model Saccharomyces cerevisiae. Our results reveal that cadmium toxicity is unexpectedly enhanced during phosphate repletion and optimal phosphate levels for yeast growth under cadmium stress conditions decline with increasing cadmium concentrations. The profound cadmium toxicity during phosphate repletion is unlikely to result from either elevated cadmium accumulation or dysregulated homeostasis of essential metals, but rather due to increased production of intracellular reactive oxygen species. We show that, under phosphate-depleted conditions, the activities of antioxidant enzymes, especially Mn-superoxide dismutase and catalase, are significantly promoted through transcriptional upregulation. Our findings highlight the important role of cellular response to phosphate limitation in mitigating cadmium toxicity and endogenous oxidative stress through the enhancement of antioxidant enzyme activity.