The sensitive and selective electrochemical detection of carcinoembryonic antigen using a nanoMIPs-aptamer sandwich assay
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Issued Date
2025-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-105021313817
Pubmed ID
41214027
Journal Title
Scientific Reports
Volume
15
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.15 No.1 (2025)
Suggested Citation
Akkapinyo C., Poo-arporn Y., Rattanakam R., Subannajui K., Lieberzeit P.A., Wolschann P., Poo-arporn R.P. The sensitive and selective electrochemical detection of carcinoembryonic antigen using a nanoMIPs-aptamer sandwich assay. Scientific Reports Vol.15 No.1 (2025). doi:10.1038/s41598-025-22971-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113149
Title
The sensitive and selective electrochemical detection of carcinoembryonic antigen using a nanoMIPs-aptamer sandwich assay
Corresponding Author(s)
Other Contributor(s)
Abstract
Carcinoembryonic antigen (CEA) is a well-established cancer biomarker that plays a crucial role in cancer diagnosis, treatment monitoring, and recurrence detection. This study presents the development of a highly sensitive and selective electrochemical sensor based on molecularly imprinted polymer nanoparticles (nanoMIPs) for CEA detection. CEA-specific nanoMIPs were synthesized and immobilized onto a screen-printed carbon electrode, providing selective recognition sites for CEA binding. The electrochemical signal probe was constructed using a metal organic framework, UiO-66-NH<inf>2</inf>, which served as the substrate for lead ion (Pb<sup>2+</sup>) adsorption and aptamer functionalization. A nanoMIPs-aptamer sandwich assay was used for CEA detection. Square wave anodic stripping voltammetry was used to measure the electrochemical response of Pb<sup>2+</sup>, which correlates with the amount of CEA captured on the electrode surface. The sensor demonstrated an excellent linear CEA detection range at concentrations between 1 and 1,000 ng/mL. The limit of detection was determined to be 1.4 ng/mL, which is below the clinical cut-off value for CEA. The proposed sandwich assay offers several advantages, including low cost, high sensitivity, good reproducibility, and excellent selectivity. When applied to CEA-spiked human serum samples with the appropriate pretreatment, the sensor achieved satisfactory recovery rates ranging from 98.12 to 103.24%, highlighting its applicability for clinical diagnostics.