Discovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET
1
Issued Date
2022-09-12
Resource Type
ISSN
14337851
eISSN
15213773
Scopus ID
2-s2.0-85132156920
Pubmed ID
35656865
Journal Title
Angewandte Chemie - International Edition
Volume
61
Issue
37
Rights Holder(s)
SCOPUS
Bibliographic Citation
Angewandte Chemie - International Edition Vol.61 No.37 (2022)
Suggested Citation
Eiamthong B., Meesawat P., Wongsatit T., Jitdee J., Sangsri R., Patchsung M., Aphicho K., Suraritdechachai S., Huguenin-Dezot N., Tang S., Suginta W., Paosawatyanyong B., Babu M.M., Chin J.W., Pakotiprapha D., Bhanthumnavin W., Uttamapinant C. Discovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET. Angewandte Chemie - International Edition Vol.61 No.37 (2022). doi:10.1002/anie.202203061 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84053
Title
Discovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET
Other Contributor(s)
Abstract
We report a bioinformatic workflow and subsequent discovery of a new polyethylene terephthalate (PET) hydrolase, which we named MG8, from the human saliva metagenome. MG8 has robust PET plastic degradation activities under different temperature and salinity conditions, outperforming several naturally occurring and engineered hydrolases in degrading PET. Moreover, we genetically encoded 2,3-diaminopropionic acid (DAP) in place of the catalytic serine residue of MG8, thereby converting a PET hydrolase into a covalent binder for bio-functionalization of PET. We show that MG8(DAP), in conjunction with a split green fluorescent protein system, can be used to attach protein cargos to PET as well as other polyester plastics. The discovery of a highly active PET hydrolase from the human metagenome—currently an underexplored resource for industrial enzyme discovery—as well as the repurposing of such an enzyme into a plastic functionalization tool, should facilitate ongoing efforts to degrade and maximize reusability of PET.