Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues
1
Issued Date
2025-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85214026360
Pubmed ID
39747178
Journal Title
Scientific Reports
Volume
15
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.15 No.1 (2025)
Suggested Citation
Suwanngam A., Schiffer P.H., Sasnarukkit A., Siripattanapipong S., Jindapunnapat K., Chinnasri B., Ruang-areerate T. Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues. Scientific Reports Vol.15 No.1 (2025). doi:10.1038/s41598-024-83214-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/102771
Title
Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues
Corresponding Author(s)
Other Contributor(s)
Abstract
Meloidogyne enterolobii, a guava root-knot nematode, is a highly virulent pest in tropical and subtropical regions causing galls or knots in roots of diverse plant species posing a serious threat to agriculture. Managing this nematode is challenging due to limitations in conventional identification based on isolation and microscopic classification requiring expertise and time. A colorimetric and fluorescent LAMP assay using simplified extraction method targeting rDNA-ITS region was developed to detect M. enterolobii DNA. The Men-LAMP assay exhibits simple procedure and achievable outcomes directly from root gall samples within 75 to 80 min, using a simplified Worm Lysis Buffer Plus (WLB +) extraction and the LAMP assay. The results could be interpreted using color and fluorescence without requiring post-amplification to minimize any possibility of contamination. The specificity showed no cross amplification with other plant-parasitic nematodes, a sensitivity was limited to 2.89 ng/μL. Our study proposes a sensitive, specific and time-efficient diagnostic tool for M. enterolobii infection as an alternative promising method for rapid and effective diagnosis at point-of-service to manage and control of M. enterolobii in export plants that can contribute to the degradation of trade restrictions and streamline of the international quarantine inspection process.