High-Throughput Bioprinting of Geometrically-Controlled Pre-Vascularized Injectable Microgels for Accelerated Tissue Regeneration
Issued Date
2023-01-01
Resource Type
ISSN
21922640
eISSN
21922659
Scopus ID
2-s2.0-85160629654
Pubmed ID
37219011
Journal Title
Advanced Healthcare Materials
Rights Holder(s)
SCOPUS
Bibliographic Citation
Advanced Healthcare Materials (2023)
Suggested Citation
Franca C.M., Athirasala A., Subbiah R., Tahayeri A., Selvakumar P., Mansoorifar A., Horsophonphong S., Sercia A., Nih L., Bertassoni L.E. High-Throughput Bioprinting of Geometrically-Controlled Pre-Vascularized Injectable Microgels for Accelerated Tissue Regeneration. Advanced Healthcare Materials (2023). doi:10.1002/adhm.202202840 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/82987
Title
High-Throughput Bioprinting of Geometrically-Controlled Pre-Vascularized Injectable Microgels for Accelerated Tissue Regeneration
Other Contributor(s)
Abstract
Successful integration of cell-laden tissue constructs with host vasculature depends on the presence of functional capillaries to provide oxygen and nutrients to the embedded cells. However, diffusion limitations of cell-laden biomaterials challenge regeneration of large tissue defects that require bulk-delivery of hydrogels and cells. Here, a strategy to bioprint geometrically controlled, endothelial and stem-cell laden microgels in high-throughput is introduced, allowing these cells to form mature and functional pericyte-supported vascular capillaries in vitro, and then injecting these pre-vascularized constructs minimally invasively in-vivo. It is demonstrated that this approach offers both desired scalability for translational applications as well as unprecedented levels of control over multiple microgel parameters to design spatially-tailored microenvironments for better scaffold functionality and vasculature formation. As a proof-of-concept, the regenerative capacity of the bioprinted pre-vascularized microgels is compared with that of cell-laden monolithic hydrogels of the same cellular and matrix composition in hard-to-heal defects in vivo. The results demonstrate that the bioprinted microgels have faster and higher connective tissue formation, more vessels per area, and widespread presence of functional chimeric (human and murine) vascular capillaries across regenerated sites. The proposed strategy, therefore, addresses a significant issue in regenerative medicine, demonstrating a superior potential to facilitate translational regenerative efforts.