Suppressive Effect of Coffee Leaves on Lipid Digestion and Absorption In Vitro
Issued Date
2024-08-01
Resource Type
eISSN
23048158
Scopus ID
2-s2.0-85200729983
Journal Title
Foods
Volume
13
Issue
15
Rights Holder(s)
SCOPUS
Bibliographic Citation
Foods Vol.13 No.15 (2024)
Suggested Citation
Sansri V., Sroyraya M., Phisalprapa P., Yosboonruang A., Ontawong A., Saokaew S., Goh B.H., Trisat K., Phewchan P., Rawangkan A., Limpeanchob N., Duangjai A. Suppressive Effect of Coffee Leaves on Lipid Digestion and Absorption In Vitro. Foods Vol.13 No.15 (2024). doi:10.3390/foods13152445 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/100479
Title
Suppressive Effect of Coffee Leaves on Lipid Digestion and Absorption In Vitro
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: Coffee leaves are a major source of bioactive components and are used as ethnomedicine. However, despite their traditional medicinal use, information about their effects on antihyperlipidemia remains limited. Methods: The aims of this study were to evaluate the main components of leaf extracts from Arabica and Robusta coffees and to examine the potential of these coffee leaves in reducing lipid digestion and absorption in vitro. Results: Coffee leaf extracts from Arabica coffee contain a high amount of caffeine, whereas extracts from Robusta coffee contain high amounts of chlorogenic acid (CGA) and caffeine. Additionally, leaf extracts from Arabica and Robusta coffee demonstrated the inhibition of pancreatic lipase, decreased micellar cholesterol solubility, and reduced bile acid binding. Furthermore, these extracts resulted in a reduction in cholesterol uptake in Caco-2 cells. Molecular docking experiments supported this discovery, showing CGA and caffeine binding to Niemann–Pick C1-like 1 (NPC1L1), a key protein in cholesterol absorption. The results indicated that CGA and caffeine can competitively bind to NPC1L1 at the cholesterol binding pocket, reducing its cholesterol binding rate. These findings suggest that coffee leaves might help suppress lipid absorption and digestion, highlighting their potential use in preventing and treating hyperlipidemia.