Evolution and virulence of porcine epidemic diarrhea virus following in vitro and in vivo propagation
Issued Date
2024-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85194550366
Journal Title
Scientific Reports
Volume
14
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.14 No.1 (2024)
Suggested Citation
Jermsutjarit P., Mebumroong S., Watcharavongtip P., Lin H., Tantituvanont A., Kaeoket K., Piñeyro P., Nilubol D. Evolution and virulence of porcine epidemic diarrhea virus following in vitro and in vivo propagation. Scientific Reports Vol.14 No.1 (2024). doi:10.1038/s41598-024-62875-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98635
Title
Evolution and virulence of porcine epidemic diarrhea virus following in vitro and in vivo propagation
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Abstract
Practice of inoculating porcine epidemic diarrhea virus (PEDV) in piglets generating feedback material might influence the genetic evolution and attenuation of PEDV. The study was conducted to evaluate evolutionary rate and attenuation following serial in vitro and in vivo propagation. In the study, PED-JPFP0-PJ, Passage 0 (P0), was isolated from infected pigs and serially passaged in Vero cells for 5 consecutive times, P1-P5. P0, P2 and P5 were then subjected to orally inoculate 3-day-old piglets. At 24 h post inoculation, intestines of each passage (F1), were collected, and subsequently sub-passaged in piglets for 2 additional passages (F2-F3). Virus titration, PEDV genomic copies number, VH:CD ratios, and immunohistochemistry were evaluated. S and ORF3 genes were characterized. The results of the study demonstrated that virus titer and virulence were negatively correlated with increased passages, both in vitro and in vivo. Increased substitution rate was observed in higher passages. The evolutionary rate of S gene was higher than that of ORF3. Seven aa changes at positions 223, 291, 317, 607, 694, 1114 and 1199, with reduced N-linked glycan were observed in P5F3. In conclusion, serial passage of PEDV, both in vitro and in vivo, influence the genetic development and the attenuation of PEDV.