Application of fungal lipase to modify palm oil by interesterification
3
Issued Date
2023
Copyright Date
1991
Language
eng
File Type
application/pdf
No. of Pages/File Size
xii, 133 p. : ill.
Access Rights
restricted access
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1991
Suggested Citation
Wimon Pheagvatanakun Application of fungal lipase to modify palm oil by interesterification. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1991. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90632
Title
Application of fungal lipase to modify palm oil by interesterification
Alternative Title(s)
การใช้ไลเปสในการดัดแปลงน้ำมันปาล์มโดยวิธีอินเตอร์เอสเตอริฟิเคชั่น
Author(s)
Advisor(s)
Abstract
Rhizopus delemar grown in a shake-flask culture consisting of 6% peptone, 3% glucose and inorganic salts (0.1% KH(,2)PO(,4);0.1% NaNO(,3) and 0.05% MgSo(,4)) for 5 days at 30 degree C produced extracellular lipase approximately 1500 unit/ml. The lipase was partially purified by ultrafiltration and CM-cellulose column chromatography and obtained lipase with specific activity 811 unit/mg protein with 16-fold purification and 50% yield. The partially purified lipase preparation showed pH optimum at 6, temperature optimum at 45 degree C, heat stability upto 45 degree C and having molecular weight 43,000. The properties of R. delemar lipase prepared in this study was similar to that of R. delemar lipase from Amano. R. delemar lipase was immobilized on various supports namely celite, pig bone powder, ENT, ENTP, PU-3 and PU-6 Lipase immobilized on pig bone powder at 50% enzyme loading show highest interesterification activity in the reaction mixture containing palm oil and stearic acid in n-hexane. Optimal conditions for lipase-catalyzed interesterification was at 35 degree C, pH 5, 6% water content when the ratio of substrates (stearic acid to palm oil) was two (wt/wt) and the enzyme content was 40% of substrate weight. The apparent Km of pig bone-adsorbed lipase for stearic acid was 2.23x10 4 M, and Vmax 0.98 mole stearic acid/ mole triglyceride/hr. The maximum percentage of stearic acid in triglyceride of palm oil obtained after 24 hr interesterification was 40-50%. The modified palm oil showed an increase in transition temperature as determined by differential scanning calorimetry.
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University