Development of a novel encystment medium: Enhancing diagnostic potential of Acanthamoeba spp.
Issued Date
2025-01-01
Resource Type
ISSN
09728988
eISSN
22310916
Scopus ID
2-s2.0-85217116057
Journal Title
Veterinary World
Volume
18
Issue
1
Start Page
110
End Page
121
Rights Holder(s)
SCOPUS
Bibliographic Citation
Veterinary World Vol.18 No.1 (2025) , 110-121
Suggested Citation
Chuprom J., Sangkanu S., Mitsuwan W., Boonhok R., Paul A.K., Rodrigues Oliveira S.M., Pereira M.L., Jimoh T.O., Rahmatullah M., Wilairatana P., Wiart C., Verma A.K., Nissapatorn V. Development of a novel encystment medium: Enhancing diagnostic potential of Acanthamoeba spp.. Veterinary World Vol.18 No.1 (2025) , 110-121. 121. doi:10.14202/vetworld.2025.110-121 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/105287
Title
Development of a novel encystment medium: Enhancing diagnostic potential of Acanthamoeba spp.
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
Walailak University
Universiti Malaysia Sabah
University of Tasmania
CICECO – Instituto de Materiais de Aveiro
Icahn School of Medicine at Mount Sinai
Catholic University of Portugal
National Institute of Tuberculosis and Respiratory Diseases
University of Development Alternative
Walailak University
Universiti Malaysia Sabah
University of Tasmania
CICECO – Instituto de Materiais de Aveiro
Icahn School of Medicine at Mount Sinai
Catholic University of Portugal
National Institute of Tuberculosis and Respiratory Diseases
University of Development Alternative
Corresponding Author(s)
Other Contributor(s)
Abstract
Background and Aim: Acanthamoeba spp. are pathogenic microorganisms linked to severe infections in humans and animals, requiring a deeper understanding of their encystation process for effective diagnostics and research. This study focused on developing a novel encystment medium to induce synchronized encystation of Acanthamoeba spp. efficiently and rapidly. Materials and Methods: The study employed response surface methodology with a central composite design to optimize the encystment medium formulation. The key components included Tris-HCl, NaCl, glucose, and MgCl2. The optimized liquid medium was spray-dried to produce a dehydrated powder for practical application. The encystation efficiency of different Acanthamoeba strains was assessed using hemocytometry and fluorescence microscopy. Results: The optimized medium, comprising 3.152 g/L Tris-HCl, 5.55 g/L NaCl, 8% (w/v) glucose, and 5.0 mM MgCl2 at pH 9.0, demonstrated exceptional encystation efficiency with rates ranging from 99% to 100%. A spray-dried powdered version of this medium was equally effective, achieving a 98.77% encystation rate for A. castellanii American Type Culture Collection 50739 in glucose-free conditions. Notably, optimal glucose concentrations varied among Acanthamoeba strains, with certain strains reaching maximum encystation at 6–8% glucose. Conclusion: This study successfully developed an innovative encystment medium that promotes rapid and efficient cyst production in Acanthamoeba spp. The medium enhances laboratory research and diagnostic capabilities, paving the way for future advancements in understanding and managing Acanthamoeba infections.
