The interferon-like proteins, Vagos, in Fenneropenaeus merguiensis elicit antimicrobial responses against WSSV and VP<inf>AHPND</inf> infection
Issued Date
2022-12-01
Resource Type
ISSN
10504648
eISSN
10959947
Scopus ID
2-s2.0-85140977073
Pubmed ID
36341873
Journal Title
Fish and Shellfish Immunology
Volume
131
Start Page
718
End Page
728
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology Vol.131 (2022) , 718-728
Suggested Citation
Limkul S., Phiwthong T., Massu A., Jaree P., Thawonsuwan J., Teaumroong N., Boonanuntanasarn S., Somboonwiwat K., Boonchuen P. The interferon-like proteins, Vagos, in Fenneropenaeus merguiensis elicit antimicrobial responses against WSSV and VP<inf>AHPND</inf> infection. Fish and Shellfish Immunology Vol.131 (2022) , 718-728. 728. doi:10.1016/j.fsi.2022.10.037 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83075
Title
The interferon-like proteins, Vagos, in Fenneropenaeus merguiensis elicit antimicrobial responses against WSSV and VP<inf>AHPND</inf> infection
Other Contributor(s)
Abstract
The Vago interferon-like protein participates in the interplay between interferon regulatory factors and the expression of immune-responsive genes. Vago was initially perceived to participate only in the antiviral activation through JAK/STAT pathway. However, certain isoforms of Vago can stimulate antimicrobial responses. Here we identify Vago isoforms in Fenneropenaeus merguiensis (FmVagos) and how they function in antiviral and antibacterial responses against highly invasive pathogens, including white spot syndrome virus (WSSV) and Vibrio parahaemolyticus (VPAHPND). Three isoforms of FmVagos were identified: FmVago4, FmVago5a, and FmVago5b, and expressed throughout tissues of the shrimp. During infection, FmVago4, FmVago5a, and FmVago5b, were up-regulated after WSSV and VPAHPND challenges at certain time points. Pre-injection of purified recombinant FmVago4 (rVago4), FmVago5a (rVago5a), and FmVago5b (rVago5b) proteins could significantly reduce the mortality of shrimp upon WSSV infection, while the increase of survival rate of VPAHPND-infected shrimp was observed only in rVago4 treatment. The immunity routes that FmVagos might instigate in response to the pathogens were examined by qRT-PCR, revealing that the JAK/STAT pathway was activated after introducing rVago4, rVago5a, and rVago5b, while the Toll/IMD pathway and proPO system, combined with PO activity, were provoked only in the rVago4-treated shrimp. Our finding suggests cross-talk between Vago's antiviral and antimicrobial responses in shrimp immunity. These findings complement previous studies in which Vago and its specific isoform could promote viral and bacterial clearance in shrimp.