A novel nested allele-specific PCR protocol for the detection of the HLA-A*33:03, a SCAR-associated allele, in Vietnamese people
Issued Date
2024-12-01
Resource Type
ISSN
0125877X
eISSN
22288694
Scopus ID
2-s2.0-85127838983
Pubmed ID
33865300
Journal Title
Asian Pacific Journal of Allergy and Immunology
Volume
42
Issue
4
Start Page
375
End Page
381
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology Vol.42 No.4 (2024) , 375-381
Suggested Citation
Pham T.T.H., Tran Q.B., Sukasem C., Nguyen V.D., Chu C.H., Do T.Q.N., Tran N.P.M., Nguyen H.H., Phung T.H. A novel nested allele-specific PCR protocol for the detection of the HLA-A*33:03, a SCAR-associated allele, in Vietnamese people. Asian Pacific Journal of Allergy and Immunology Vol.42 No.4 (2024) , 375-381. 381. doi:10.12932/ap-201120-1000 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/103087
Title
A novel nested allele-specific PCR protocol for the detection of the HLA-A*33:03, a SCAR-associated allele, in Vietnamese people
Author's Affiliation
VinUniversity
Ramathibodi Hospital
National Institute of Hygiene and Epidemiology Hanoi
Bach Mai Hospital
Hanoi College of Pharmacy
National Institute of Nutrition Vietnam
Vietnam Academy of Science and Technology
Vinmec Times City International Hospital
The Thai Severe Cutaneous Adverse Drug Reaction (THAI-SCAR) Research Group
Ramathibodi Hospital
National Institute of Hygiene and Epidemiology Hanoi
Bach Mai Hospital
Hanoi College of Pharmacy
National Institute of Nutrition Vietnam
Vietnam Academy of Science and Technology
Vinmec Times City International Hospital
The Thai Severe Cutaneous Adverse Drug Reaction (THAI-SCAR) Research Group
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: Severe cutaneous adverse drug reactions (SCARs) are rare but deadly drug reactions with severe damages to patients. One of the most well-known SCARs risk factors is the human leukocyte antigen (HLA) genes polymorphism. Among the HLA polymorphic alleles, the HLA-A*33:03 allele has been found in association with SCARs induced by various drugs, especially in Asian people. There has not been any report on the specific detection protocol of the HLA-A*33:03 allele. Objective: This study aimed to design a nested AS-PCR protocol for detecting and distinguishing diplotype genotype of the HLA-A*33:03 allele. Methods: A nested allele-specific (AS)-PCR protocol with four primer sets was designed. The method was compared with the Sanger sequencing method on 100 samples of unknown genotypes of unrelated Vietnamese people. Results: The nested AS-PCR method could identify the HLA-A*33:03 allele and the HLA-A*33:03 diplotype genotypes. Comparison with the Sanger sequencing method showed an absolute agreement (ĸ = 1.00, p < 0.001). The nested AS-PCR protocol had a sensitivity of 100% (95%CI: 92.13-100%) and a specificity of 100% (95%CI: 93.51-100%). The protocol was used for the determination of HLA-A*33:03 allele distribution in 810 unrelated Vietnamese Kinh people, showing a frequency of HLA-A*33:03 carriers of 19.6% and an allele frequency of 10.55%. Conclusions: A novel nested AS-PCR method with a hundred-percent sensitivity and a specificity for the HLA-A*33:03 allele detection was reported. The protocol can be applied for the stratification of patients at SCAR risks with various drugs.