Ultrathin Cu-TCPP Nanosheet-Based Electrochemical Microsensor for Detecting the Immunosuppressive Drug Mycophenolic Acid
Issued Date
2023-12-22
Resource Type
eISSN
25740970
Scopus ID
2-s2.0-85180084217
Journal Title
ACS Applied Nano Materials
Volume
6
Issue
24
Start Page
22979
End Page
22988
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Applied Nano Materials Vol.6 No.24 (2023) , 22979-22988
Suggested Citation
Deng M., Jin W., Yang W., Tian L., Gao X., Wang X., Feng L., Janyasupab M., Zhou B., Zhang Y. Ultrathin Cu-TCPP Nanosheet-Based Electrochemical Microsensor for Detecting the Immunosuppressive Drug Mycophenolic Acid. ACS Applied Nano Materials Vol.6 No.24 (2023) , 22979-22988. 22988. doi:10.1021/acsanm.3c04334 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/95591
Title
Ultrathin Cu-TCPP Nanosheet-Based Electrochemical Microsensor for Detecting the Immunosuppressive Drug Mycophenolic Acid
Corresponding Author(s)
Other Contributor(s)
Abstract
Mycophenolic acid (MPA), which is a potent immunosuppressant, has been widely used to prevent organ rejection after organ transplantation. In clinical practice, it is essential to monitor the free MPA concentration. Herein, convenient and fast electrochemical microsensing technology is developed for directly detecting the MPA concentration. To implement this method, ultrathin copper-based metal-organic framework (Cu-MOF) nanosheets with a thickness of about 6.6 nm are used to detect MPA. An analysis of electrochemical behavior reveals the direct electrochemical catalytic oxidation mechanism of MPA molecules on a Cu(II) tetrakis(4-carboxyphenyl)porphyrin (Cu-TCPP)-modified electrode. Furthermore, electrochemical testing results show that the ultrathin Cu-TCPP nanosheet-based microsensor exhibits high sensitivity and specificity for MPA in the nano- to micromolar range, with a detection limit of 10 nM. X-ray absorption spectroscopy (XAS) characterization reveals that the unsaturated metal ions in ultrathin nanosheets are the active sites responsible for boosting the catalytic oxidation of MPA molecules. High-performance liquid chromatography-tandem mass spectrometry further shows the catalytic oxidation product of MPA and that the phenolic hydroxyl group in the MPA molecule is the active site where the catalytic reaction occurs. The considerable sensing performance of the Cu-TCPP nanosheet-based microsensor proves that it is possible to develop a fast and convenient method for the direct high-frequency monitoring of immunosuppressive drugs.