A new coumarin-based “OFF–ON” fluorescent sensor for H<inf>2</inf>S detection in HeLa cells
Issued Date
2025-02-05
Resource Type
ISSN
13861425
Scopus ID
2-s2.0-85204949472
Pubmed ID
39342725
Journal Title
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume
326
Rights Holder(s)
SCOPUS
Bibliographic Citation
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Vol.326 (2025)
Suggested Citation
Sontisiri P., Promrug D., Arthan D., Choengchan N., Thongyoo P. A new coumarin-based “OFF–ON” fluorescent sensor for H<inf>2</inf>S detection in HeLa cells. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Vol.326 (2025). doi:10.1016/j.saa.2024.125170 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102756
Title
A new coumarin-based “OFF–ON” fluorescent sensor for H<inf>2</inf>S detection in HeLa cells
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Corresponding Author(s)
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Abstract
A new “OFF–ON” coumarin-based fluorescent probe for H2S detection was designed and successfully developed through O-sulfonylation between a dabsyl quencher and 7-hydroxy-4-methylcoumarin as a fluorescent reporter, based on a FRET approach. This H2S responsive probe, utilizing H2S assisted thiolysis of a sulfonate ester as the sensing strategy, demonstrated excellent performance towards H2S with a limit of detection (LoD) of 1.64 µM, along with superb selectivity, good stability and high specificity towards H2S without interference from other biomarkers and analytes. Moreover, dabsyl-7-hydroxy-4-methylcoumarin (dabsylcoumarin) is capable of permeating the cell membrane and effectively visualizing the level of H2S in the living HeLa cells without cytotoxicity.