microRNA profiling of exosomes derived from plasma and their potential as biomarkers for Opisthorchis viverrini-associated cholangiocarcinoma
7
Issued Date
2024-10-01
Resource Type
ISSN
0001706X
eISSN
18736254
Scopus ID
2-s2.0-85201927111
Pubmed ID
39151716
Journal Title
Acta Tropica
Volume
258
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Tropica Vol.258 (2024)
Suggested Citation
Supradit K., Wongprasert K., Tangphatsornruang S., Yoocha T., Sonthirod C., Pootakham W., Thitapakorn V., Butthongkomvong K., Phanaksri T., Kunjantarachot A., Klongprateeppon H., Sattavacharavech P., Prasopdee S. microRNA profiling of exosomes derived from plasma and their potential as biomarkers for Opisthorchis viverrini-associated cholangiocarcinoma. Acta Tropica Vol.258 (2024). doi:10.1016/j.actatropica.2024.107362 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/100647
Title
microRNA profiling of exosomes derived from plasma and their potential as biomarkers for Opisthorchis viverrini-associated cholangiocarcinoma
Corresponding Author(s)
Other Contributor(s)
Abstract
Cholangiocarcinoma (CCA) is a life-threatening disease that impacts patients worldwide. In Southeast Asian countries, the liver fluke Opisthorchis viverrini plays a major role in inducing carcinogenesis of the bile ducts. Due to its asymptomatic nature, O. viverrini infections are rarely treated, consequently leading to the development of advanced stages of CCA before diagnosis. Despite the current use of exosomal microRNAs (miRNA) as diagnostic biomarkers for the early detection of many types of cancer, the applications for miRNA remain limited with CCA. Circulating exosomes, membranous vesicles essential for intercellular communication, were found to contain unique miRNA. In this study, we conducted next-generation sequencing (Ion Torrent PGM) and bioinformatics to characterize and compare the contents of exosomal miRNA derived from the plasma of CCA patients, O. viverrini-infected patients, and healthy individuals, as well as to identify and validate key molecules as markers for screening the diagnosis of CCA and O. viverrini infection. The obtained results showed the success of using NGS technology in discovering exosomal miRNAs, specifically miR-194–5p and miR-192–5p, both of which were upregulated in the O. viverrini-infected group. Interestingly, miR-192–5p was upregulated while miR-194–5p was downregulated in CCA, suggesting their potential use as biomarkers for screening CCA and O. viverrini infection, especially in O. viverrini-endemic areas.