Kinetic study of D-galactosamine, 6-azauridine and 5-fluorouridine in chemotherapy of hepatoma
Issued Date
1987
Copyright Date
1987
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
79 leaves. : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Pathobiology))--Mahidol University, 1987
Suggested Citation
Yaowapa Maneerat Kinetic study of D-galactosamine, 6-azauridine and 5-fluorouridine in chemotherapy of hepatoma. Thesis (M.Sc. (Pathobiology))--Mahidol University, 1987. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/103374
Title
Kinetic study of D-galactosamine, 6-azauridine and 5-fluorouridine in chemotherapy of hepatoma
Alternative Title(s)
การศึกษา kinetic ของยา D-galactosamine, 6-azauridine และ 5-fluorouridine ในการรักษามะเร็งตับ
Author(s)
Advisor(s)
Abstract
.6-AzaUrd, 5-FUrd and Urd in ascitic fluid and AS-30D cells were analysed by reversed phase HPLC system and detected with coupled UV spectrophotometre. The concentration determined by peak-height analysis were used for kinetic analysis of drugs. The studies with AS-30D ascitic hepatoma cells in vivo revealed the rapid increase of 6-azaUrd or 5-FUrd level in both ascitic fluid and cells after administration of the respective drugs. The exponential decrease was observed after 30 min of administration for both drugs in ascitic fluid. In cells, 5-FUrd level declined rather sharploy, whereas the level of 6-azaUrd decreased at slower rate. Pretreatment with GalN and 6-azaUrd significantly accerelated the rate of reduction of 5-FUrd in both ascitic fluid and cells. In addtition, pretreatment was followed by UTP depletion to the minimum level within 60 to 90 min. This interval seems to be the most suitable time for sequential administration of 5-FUrd in chemotherapy. Experimental chemotherapy of intrahepatic tumor bearing rat was performed with doses of drugs adjusted from the kinetic study. The schedule employed was GalN (100 mg/kg) and 6-azaUrd (30 mg/kg) at O min and 5-FUrd (15 mg/kg) at 60 min later. The treatment was started on the fifth day after transplantation and every second day, totally 7 times. The tumor bearing rats receiving GalN, 6-azaUrd and 5-FUrd in combination as scheduled had an extended median survival time over the untreated and the animals treated with other regimens.
Description
Pathobiology (Mahidol University 1987)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Pathobiology
Degree Grantor(s)
Mahidol University