A discrete dielectrophoresis device for the separation of malaria-infected cells
5
Issued Date
2022-06-01
Resource Type
ISSN
01730835
eISSN
15222683
Scopus ID
2-s2.0-85128753697
Pubmed ID
35338790
Journal Title
Electrophoresis
Volume
43
Issue
12
Start Page
1347
End Page
1356
Rights Holder(s)
SCOPUS
Bibliographic Citation
Electrophoresis Vol.43 No.12 (2022) , 1347-1356
Suggested Citation
Panklang N., Techaumnat B., Wisitsoraat A., Putaporntip C., Chotivanich K., Suzuki Y. A discrete dielectrophoresis device for the separation of malaria-infected cells. Electrophoresis Vol.43 No.12 (2022) , 1347-1356. 1356. doi:10.1002/elps.202100271 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83726
Title
A discrete dielectrophoresis device for the separation of malaria-infected cells
Other Contributor(s)
Abstract
Malaria is a serious disease caused by Plasmodium parasites that infect red blood cells (RBCs). This paper presents the continuous separation of malaria-infected RBCs (iRBCs) from normal blood cells. The proposed method employed the discrete dielectrophoresis (DEP) in a microfluidic device with interdigitated electrodes. Our aim is to treat a sample having high concentration of cells to realize high throughput and to prevent the clogging of the microchannel with the use of the discrete DEP. The discrete DEP force for deflecting cells in the device was controlled by adjusting the magnitude, frequency, and duty cycle of the applied voltage. The effectiveness of the proposed method was demonstrated by separating the malaria-infected cells in samples having a cell concentration of 106 cells/µl. From experimental results, we determined the enrichment that is needed to enhance the detection in the case of low parasitemia. The enrichment of the infected cells at the device output was 3000 times as high as that of the input containing 1 infected cell to 106 normal cells. Therefore, the proposed method is highly effective and can significantly facilitate the detection of the infected cells for the identification of Malaria patients.