Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand
Issued Date
2022-07-01
Resource Type
ISSN
19957645
Scopus ID
2-s2.0-85136795663
Journal Title
Asian Pacific Journal of Tropical Medicine
Volume
15
Issue
7
Start Page
314
End Page
321
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Tropical Medicine Vol.15 No.7 (2022) , 314-321
Suggested Citation
Surasiang T., Chumkiew S., Martviset P., Chantree P., Jamklang M. Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand. Asian Pacific Journal of Tropical Medicine Vol.15 No.7 (2022) , 314-321. 321. doi:10.4103/1995-7645.351763 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87279
Title
Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand
Other Contributor(s)
Abstract
Objective: To determine the prevalence of mosquito larvae in campus areas and the infection rate of endosymbiotic bacteria, Wolbachia in mosquito larvae. Method: The mosquito larvae samples were collected in residential areas and academic buildings of Suranaree University of Technology located in Northeastern Thailand during 2017-2018. Mosquito species identification was performed using GLOBE mosquito protocols and Rattanarithikul & Panthusiri's keys. The gene encoding for the surface protein of Wolbachia was amplified by PCR and confirmed by DNA sequencing. Results: Armigeres sp. is the highest proportion of mosquito larvae followed by Culex spp., Aedes albopictus, Aedes aegypti, and Toxorynchites spp., respectively. Aedes aegypti have breeding sites mostly in the containers found indoors, whereas the main breeding sites of Aedes albopictus were found in both outdoors and indoors. The House Index and Breteau Index for Aedes spp. was more than 5% and 20%, respectively, in both areas, indicating that these areas are dengue sensitive. The highest proportion of Wolbachia infection was found in the larvae of Culex spp. (86.21%), followed by Aedes albopictus (69.23%) and rarely detected in Aedes aegypti (9.09%). Conclusion: The present study reported the first natural infection of Wolbachia in mosquito larvae in Thailand. Our result suggested that the mosquito species containing higher proportion of Wolbachia are less likely to be vectors for dengue. Therefore, Wolbachia transfection in mosquito larvae could be applied as a biocontrol for dengue and other mosquito-borne disease prevention.