Dengue virus harnesses mosquito Syntenin to load and secrete viral RNA into salivary exosomes
Issued Date
2026-03-24
Resource Type
eISSN
10916490
Scopus ID
2-s2.0-105033551730
Pubmed ID
41838911
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume
123
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
Proceedings of the National Academy of Sciences of the United States of America Vol.123 No.12 (2026) , e2520697123
Suggested Citation
Rachenne F., Schneider N., Rey-Cadilhac F., Serrato-Pomar I., Pruvost L., Pietro L.D., Dainat J., Vernet A., Cazevieille C., Ancelin A., Him J.L.K., Jansen S., Miot E.F., Fraering J., Simon L., Missé D., Morille M., Hammann P., Seveno M., Urbach S., Dallmeier K., Marois É., Pompon J. Dengue virus harnesses mosquito Syntenin to load and secrete viral RNA into salivary exosomes. Proceedings of the National Academy of Sciences of the United States of America Vol.123 No.12 (2026) , e2520697123. doi:10.1073/pnas.2520697123 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116033
Title
Dengue virus harnesses mosquito Syntenin to load and secrete viral RNA into salivary exosomes
Author's Affiliation
CNRS Centre National de la Recherche Scientifique
Université de Montpellier
Chiang Mai University
Rega Institute for Medical Research
Institut Charles Gerhardt Montpellier
Hôpital Saint Eloi
Institut de Biologie Moléculaire et Cellulaire
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Institute of Molecular Biosciences, Mahidol University
BioCampus Montpellier
Université de Montpellier
Chiang Mai University
Rega Institute for Medical Research
Institut Charles Gerhardt Montpellier
Hôpital Saint Eloi
Institut de Biologie Moléculaire et Cellulaire
Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle
Institute of Molecular Biosciences, Mahidol University
BioCampus Montpellier
Corresponding Author(s)
Other Contributor(s)
Abstract
Viruses exploit extracellular vesicles (EVs) to transfer infection-enhancing viral RNAs. However, mechanisms underlying viral RNA loading remain elusive. We leveraged our previous discovery that dengue virus secretes transmission-enhancing subgenomic flaviviral RNA (sfRNA) into mosquito salivary EVs to investigate viral RNA loading mechanism. We demonstrate that sfRNA alone promotes the secretion of sfRNA-containing EVs marked by the mosquito EV biogenesis protein AeSyntenin, by applying microscopy and viral genetic editing in in vitro and in vivo models. SfRNA via its stem loop structures interacts intracellularly with mosquito AeSyntenin and this interaction is selectively maintained within EVs as shown by complementary RNA-affinity chromatography and RNA immunoprecipitation, and AI-based prediction. Finally, we used systemic and salivary gland-specific protein depletion to establish a functional role for mosquito AeSyntenin in exosome production and salivary secretion of sfRNA. We propose that sfRNA binds AeSyntenin to drive its selective packaging and release into exosomes, elucidating a mechanism for viral RNA incorporation into EVs.