Plumbagin Inhibits Cadmium-Induced Interleukin-6/STAT3 Signaling in the Triple-Negative Breast Cancer Cell Line
Issued Date
2025-02-01
Resource Type
eISSN
2476762X
Scopus ID
2-s2.0-86000190199
Pubmed ID
40022690
Journal Title
Asian Pacific journal of cancer prevention : APJCP
Volume
26
Issue
2
Start Page
465
End Page
470
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific journal of cancer prevention : APJCP Vol.26 No.2 (2025) , 465-470
Suggested Citation
Titiwattanakarn T., Settacomkul R., Kasemsuk T., Vivithanaporn P. Plumbagin Inhibits Cadmium-Induced Interleukin-6/STAT3 Signaling in the Triple-Negative Breast Cancer Cell Line. Asian Pacific journal of cancer prevention : APJCP Vol.26 No.2 (2025) , 465-470. 470. doi:10.31557/APJCP.2025.26.2.465 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/106697
Title
Plumbagin Inhibits Cadmium-Induced Interleukin-6/STAT3 Signaling in the Triple-Negative Breast Cancer Cell Line
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Corresponding Author(s)
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Abstract
INTRODUCTION: Plumbagin has been found to reduce proinflammatory cytokine expression in activated macrophages and carrageenan-induced paw edema. Cadmium triggers the release of interleukin-6 (IL-6), a key mediator of inflammation and carcinogenesis in many cell types. The effects of plumbagin on cadmium-induced inflammation in triple-negative breast cancer cells are unknown. METHOD: We investigated the effects of plumbagin on cadmium-induced IL-6 expression and signal transducer and activator of transcription 3 (STAT3) activation in MDA-MB-231, a triple-negative breast cancer cell line, using real-time PCR, ELISA, and Western blotting. RESULT: Non-cytotoxic concentrations of cadmium chloride at 1 and 10 μM upregulated the IL-6 mRNA expression after 3 h of exposure and increased the IL-6 release after 24 h. Plumbagin at 4 μM or more was toxic to cells after 24 h. Plumbagin at 1 μM co-treated with cadmium reduced the expression and secretion of IL-6. At 24-h post-exposure, plumbagin decreased the levels of phosphorylated STAT3 induced by cadmium. CONCLUSION: Plumbagin inhibits cadmium-induced IL-6/STAT3 signaling in a triple-negative breast cancer cell and further in vivo studies are required to elucidate the potential use of plumbagin on cancer progression.