Functional characterization of the recombinant L-type lectin from red alga Gracilaria fisheri with antibacterial potential
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Issued Date
2025-07-01
Resource Type
ISSN
22119264
Scopus ID
2-s2.0-105004812718
Journal Title
Algal Research
Volume
89
Rights Holder(s)
SCOPUS
Bibliographic Citation
Algal Research Vol.89 (2025)
Suggested Citation
Boonsri B., Rudtanatip T., Khunrare P., Rattanarojpong T., Withyachumnarnkul B., Wongprasert K. Functional characterization of the recombinant L-type lectin from red alga Gracilaria fisheri with antibacterial potential. Algal Research Vol.89 (2025). doi:10.1016/j.algal.2025.104092 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/110270
Title
Functional characterization of the recombinant L-type lectin from red alga Gracilaria fisheri with antibacterial potential
Corresponding Author(s)
Other Contributor(s)
Abstract
Marine algal lectins are promising bioactive compounds with antimicrobial potential. This study produced and characterized a recombinant legume-type lectin from Gracilaria fisheri (rLGFL) and evaluated its antibacterial activity. The LGFL gene was cloned, sequence-verified, and expressed in Escherichia coli. Western blot analysis using a mouse polyclonal antiserum confirmed rLGFL expression. Functional assays demonstrated strong hemagglutination against rabbit erythrocytes, indicating carbohydrate recognition domain (CRD) functionality. rLGFL agglutinated Vibrio parahaemolyticus (3HP), Vibrio harveyi (1114), and Aeromonas veronii while exhibiting bactericidal activity against all tested pathogens, including non-agglutinated V. parahaemolyticus (A3212), Streptococcus agalactiae, Aeromonas hydrophila, and Edwardsiella ictaluri. These findings suggest dual antibacterial mechanisms: agglutination-mediated pathogen immobilization and direct bactericidal effects. Seasonal analysis of G. fisheri from Songkhla, Thailand, revealed peak LGFL expression from January to March and July to September, identifying optimal harvesting periods. This study highlights rLGFL as a potential eco-friendly antibacterial agent with applications in aquaculture and disease management.