Cloning of rec A gene from Xanthomonas oryzae isolate 8707
1
Issued Date
2024
Copyright Date
1991
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
v, 85 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Microbiology))--Mahidol University, 1991
Suggested Citation
Siritida Rabibhadana Cloning of rec A gene from Xanthomonas oryzae isolate 8707. Thesis (M.Sc. (Microbiology))--Mahidol University, 1991. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/99057
Title
Cloning of rec A gene from Xanthomonas oryzae isolate 8707
Alternative Title(s)
การศึกษายีน rec A ของเชื้อ Xanthomanas oryzae isolate 8707
Author(s)
Advisor(s)
Abstract
RecA-like gene of Xanthomonas oryzae isolate 8707 chromosomal DNA have been cloned by using rationale of complementation E. coli recA-mutation and selected for mitomycin C resistant phenotype. Four transformants from six thousand colonies were retransformed into E. coli DH5a, HB101 and BW368 and checked for MMS and mitomycin C resistant. Result from Southern blot analysis of one of these transformants named pSM-A1 confirmed that the insert portion of this clone derived from Xanthomonas oryzae isolate 8707 chromosomal DNA. Significantly, from immunological analysis antiserum raised against E. coli RecA protein cross-reacted with Xanthomonas phaseolin, Xanthomonas oryzae 8707, Xanthonmonas campestris pv campestris. Furthermore, it also cross-reacted with cell lysate from E. coli BW368 harboring pSM-A1 but it did not react with cell lysate from E. coli BW368 RecA protein, A single band detected from pSM A1 cell lysate with an apparent molecular weight nearly equal to a single band detected from Xanthomonas oryzae isolate 8707 cell lysate and a protein band molecular weight 40,000 E. coli protein, These results indicated that pSM-Al clone contains Xanthomonas oryzae isolate 8707 recA-like gene.
Description
Microbiology (Mahidol University 1991)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Microbiology
Degree Grantor(s)
Mahidol University