Melatonin attenuates dimethyl sulfoxide– and Zika virus–induced degeneration of porcine induced neural stem cells
Issued Date
2022-03-01
Resource Type
ISSN
10712690
eISSN
1543706X
Scopus ID
2-s2.0-85125547339
Pubmed ID
35235152
Journal Title
In Vitro Cellular and Developmental Biology - Animal
Volume
58
Issue
3
Start Page
232
End Page
242
Rights Holder(s)
SCOPUS
Bibliographic Citation
In Vitro Cellular and Developmental Biology - Animal Vol.58 No.3 (2022) , 232-242
Suggested Citation
Horcharoensuk P., Yang-en S., Chakritbudsabong W., Samatiwat P., Pramong R., Rungarunlert S., Rungsiwiwut R. Melatonin attenuates dimethyl sulfoxide– and Zika virus–induced degeneration of porcine induced neural stem cells. In Vitro Cellular and Developmental Biology - Animal Vol.58 No.3 (2022) , 232-242. 242. doi:10.1007/s11626-022-00648-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83805
Title
Melatonin attenuates dimethyl sulfoxide– and Zika virus–induced degeneration of porcine induced neural stem cells
Author's Affiliation
Other Contributor(s)
Abstract
Domestic pigs have become increasingly popular as a model for human diseases such as neurological diseases. Drug discovery platforms have increasingly been used to identify novel compounds that combat neurodegeneration. Currently, bioactive molecules such as melatonin have been demonstrated to offer a neuroprotective effect in several studies. However, a neurodegenerative platform to study novel compounds in a porcine model has not been fully established. In this study, characterized porcine induced neural stem cells (iNSCs) were used for evaluation of the protective effect of melatonin against chemical and pathogenic stimulation. First, the effects of different concentrations of melatonin on the proliferation of porcine iNSCs were studied. Second, porcine iNSCs were treated with the appropriate concentration of melatonin prior to induced degeneration with dimethyl sulfoxide or Zika virus (ZIKV). The results demonstrated that the percentages of Ki67 expression in porcine iNSCs cultured in 0.1, 1, and 10 nM melatonin were not significantly different from that in the control groups. Melatonin at 1 nM protected porcine iNSCs from DMSO-induced degeneration, as confirmed by a dead cell exclusion assay and mitochondrial membrane potential (ΔΨm) analysis. In addition, pretreatment with melatonin reduced the percentage of dead porcine iNSCs after ZIKV infection. Melatonin increased the ΔΨm, resulting in a decrease in cell degeneration. However, pretreatment with melatonin was unable to suppress ZIKV replication in porcine iNSCs. In conclusion, the present study demonstrated the anti-degenerative effect of melatonin against DMSO- and ZIKV-induced degeneration in porcine iNSCs.