Role of Dietary Factors on DNA Methylation Levels of TNF-Alpha Gene and Proteome Profiles in Obese Men
Issued Date
2024-03-01
Resource Type
eISSN
20726643
Scopus ID
2-s2.0-85188946157
Journal Title
Nutrients
Volume
16
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nutrients Vol.16 No.6 (2024)
Suggested Citation
Boonrong C., Roytrakul S., Shantavasinkul P.C., Sritara P., Sirivarasai J. Role of Dietary Factors on DNA Methylation Levels of TNF-Alpha Gene and Proteome Profiles in Obese Men. Nutrients Vol.16 No.6 (2024). doi:10.3390/nu16060877 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97865
Title
Role of Dietary Factors on DNA Methylation Levels of TNF-Alpha Gene and Proteome Profiles in Obese Men
Corresponding Author(s)
Other Contributor(s)
Abstract
Integrated omics-based platforms from epigenomics and proteomics technologies are used to identify several important mechanisms in obesity etiology, food components, dietary intake, regulation of biological pathways, and potential new intervention targets. Therefore, this study aimed to analyze whether dietary factors involved in the methylation of tumor necrosis factor (TNF)-α are implicated in differential protein expression in people with normal weight and obesity. Methods: The participants were classified into the non-obese (N = 100) and obese (N = 133) groups. DNA methylation levels of the TNF-alpha gene and proteomics were analyzed using the pyrosequencing method and LC-MS-MS, respectively. Results: Comparison between geometric means of DNA methylation of TNF-α showed lower levels in subjects with obesity than in those without obesity (p < 0.05). There were associations between dietary factors and some metabolic syndrome components and TNF-α DNA methylation levels. Proteomic analysis showed important signaling pathways related to obesity, with 95 significantly downregulated proteins and 181 upregulated proteins in the non-obese group compared with the obese group. Conclusion: This study shows an association between the dietary factors involved in the methylation of TNF-α and differential protein expression related to obesity. However, a large sample size in future studies is required to confirm our results.