Insights into the Mechanism of Pre-mRNA Splicing of Tiny Introns from the Genome of a Giant Ciliate Stentor coeruleus
Issued Date
2022-09-01
Resource Type
ISSN
16616596
eISSN
14220067
Scopus ID
2-s2.0-85138381569
Pubmed ID
36142882
Journal Title
International Journal of Molecular Sciences
Volume
23
Issue
18
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences Vol.23 No.18 (2022)
Suggested Citation
Nuadthaisong J., Phetruen T., Techawisutthinan C., Chanarat S. Insights into the Mechanism of Pre-mRNA Splicing of Tiny Introns from the Genome of a Giant Ciliate Stentor coeruleus. International Journal of Molecular Sciences Vol.23 No.18 (2022). doi:10.3390/ijms231810973 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83619
Title
Insights into the Mechanism of Pre-mRNA Splicing of Tiny Introns from the Genome of a Giant Ciliate Stentor coeruleus
Author's Affiliation
Other Contributor(s)
Abstract
Stentor coeruleus is a ciliate known for its regenerative ability. Recent genome sequencing reveals that its spliceosomal introns are exceptionally small. We wondered whether the multimegadalton spliceosome has any unique characteristics for removal of the tiny introns. First, we analyzed intron features and identified spliceosomal RNA/protein components. We found that all snRNAs are present, whereas many proteins are conserved but slightly reduced in size. Some regulators, such as Serine/Arginine-rich proteins, are noticeably undetected. Interestingly, while most parts of spliceosomal proteins, including Prp8′s positively charged catalytic cavity, are conserved, regions of branching factors projecting to the active site are not. We conjecture that steric-clash avoidance between spliceosomal proteins and a sharply looped lariat might occur, and splicing regulation may differ from other species.