Sublethal infection of C3H/HeNJ against Leptospira interrogans serovar Pomona
3
Issued Date
2023-02-01
Resource Type
ISSN
0001706X
eISSN
18736254
Scopus ID
2-s2.0-85145695011
Pubmed ID
36216095
Journal Title
Acta Tropica
Volume
238
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Tropica Vol.238 (2023)
Suggested Citation
Krangvichian P., Nakornpakdee Y., Sangjun N., Komanee P., Techawiwattanaboon T., Patarakul K. Sublethal infection of C3H/HeNJ against Leptospira interrogans serovar Pomona. Acta Tropica Vol.238 (2023). doi:10.1016/j.actatropica.2022.106701 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81461
Title
Sublethal infection of C3H/HeNJ against Leptospira interrogans serovar Pomona
Other Contributor(s)
Abstract
Leptospirosis is a worldwide zoonotic disease caused by pathogenic Leptospira spp. Leptospires can infect a variety of mammalian species. Golden Syrian hamsters are mostly used to study acute leptospirosis. However, the immunopathogenic mechanism is poorly understood because immunological reagents for hamsters are limited. This study aimed to establish C3H/HeNJ mice as an animal model for leptospirosis. Five-week-old C3H/HeNJ mice were infected with either low (103 cells) or high (106 cells) inoculum dose of Leptospira interrogans serovar Pomona. All mice were investigated for survival rate, leptospiral load and histopathology of target organs, antibody levels, and cytokine production (IFN-γ, IL-6 and IL-10) at day 28 post-infection. All infected mice survived and did not develop acute lethal infection. However, C3H/HeNJ mice infected with 106 cells of leptospires showed kidney colonization of leptospires and pathological changes in the lung and kidney including renal fibrosis. The glomerular size in PAS-D stained kidney tissues of C3H/HeNJ mice infected with 106 cells of leptospires was significantly reduced compared to that of mice infected with 103 cells of leptospires and non-infected mice. High-dose leptospires induced significantly greater levels of IFN-gamma and IL-6 than low-dose leptospires, but IL-10 level was not significantly different. Moreover, 106 leptospiral cells induced predominant IgG2a isotype suggesting Th1-like response. These results suggest that C3H/HeNJ mice may be used as a sublethal model of leptospirosis.