A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia
Issued Date
2023-12-01
Resource Type
eISSN
25219855
Scopus ID
2-s2.0-85184101314
Pubmed ID
38308826
Journal Title
Tropical biomedicine
Volume
40
Issue
4
Start Page
400
End Page
405
Rights Holder(s)
SCOPUS
Bibliographic Citation
Tropical biomedicine Vol.40 No.4 (2023) , 400-405
Suggested Citation
Gao Y., Du J.G., Jirapattharasate C., Galon E., Ji S.W., Ran Z.G., Xia Y.Q. A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia. Tropical biomedicine Vol.40 No.4 (2023) , 400-405. 405. doi:10.47665/tb.40.4.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97168
Title
A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia
Corresponding Author(s)
Other Contributor(s)
Abstract
Beta toxin (CPB) is a lethal toxin and plays a key role in enterotoxemia of ruminants caused by Clostridium perfringens type C strain. The existing vaccines based on crude CPB need time-consuming detoxification and difficult quality control steps. In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge. These data suggest that genetically detoxified rSUMO-CPBm4 is a promising subunit vaccine candidate for C. perfringens type C beta enterotoxemia.