Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) hybrid nanomaterials as immunoassay label for low-density lipoprotein detection
2
Issued Date
2022-02-01
Resource Type
ISSN
00263672
eISSN
14365073
Scopus ID
2-s2.0-85123751917
Pubmed ID
35075569
Journal Title
Microchimica Acta
Volume
189
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microchimica Acta Vol.189 No.2 (2022)
Suggested Citation
Prakobkij A., Jarujamrus P., Chunta S., Chawengkirttikul R., Keawin T., Malahom N., Tamuang S., Amatathongchai M., Citterio D. Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) hybrid nanomaterials as immunoassay label for low-density lipoprotein detection. Microchimica Acta Vol.189 No.2 (2022). doi:10.1007/s00604-022-05173-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84209
Title
Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) hybrid nanomaterials as immunoassay label for low-density lipoprotein detection
Author's Affiliation
Other Contributor(s)
Abstract
Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) are demonstrated as superior peroxidase mimic antibody labels alternative to horseradish peroxidase (HRP) in an immunoassay, potentially overcoming some of the inherent disadvantages of HRP and other enzyme mimicking nanomaterials. They revealed efficient peroxidase-like activity and catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to form the intense blue product (at 620 nm) in the presence of hydrogen peroxide (H2O2). Using low-density lipoprotein (LDL) as a model target, an ultra-low limit of detection (0.0051 mg/dL) and a linear range of 0.0625–0.750 mg/dL were achieved, exhibiting higher sensitivity than the HRP-based immunoassay. Thus, the proposed N-CDs/Ni-MnFe-LDHs can be used as HRP mimicking analogs for developing highly sensitive colorimetric immunosensors for detection of biomarkers, as well as trace chemical analysis. Graphical abstract: [Figure not available: see fulltext.]