Activity of a Bacteriophage Cocktail to Control Salmonella Growth Ex Vivo in Avian, Porcine, and Human Epithelial Cell Cultures
9
Issued Date
2023-03-01
Resource Type
ISSN
26416530
eISSN
26416549
Scopus ID
2-s2.0-85151511962
Journal Title
PHAGE: Therapy, Applications, and Research
Volume
4
Issue
1
Start Page
11
End Page
25
Rights Holder(s)
SCOPUS
Bibliographic Citation
PHAGE: Therapy, Applications, and Research Vol.4 No.1 (2023) , 11-25
Suggested Citation
Nale J.Y., Ahmed B., Haigh R., Shan J., Phothaworn P., Thiennimitr P., Garcia A., Abuoun M., Anjum M.F., Korbsrisate S., Galyov E.E., Malik D.J., Clokie M.R.J. Activity of a Bacteriophage Cocktail to Control Salmonella Growth Ex Vivo in Avian, Porcine, and Human Epithelial Cell Cultures. PHAGE: Therapy, Applications, and Research Vol.4 No.1 (2023) , 11-25. 25. doi:10.1089/phage.2023.0001 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/82647
Title
Activity of a Bacteriophage Cocktail to Control Salmonella Growth Ex Vivo in Avian, Porcine, and Human Epithelial Cell Cultures
Other Contributor(s)
Abstract
We examined the activity of phages to control the growth of chicken and swine Salmonella strains in avian (CHIC-8E11), porcine (IPEC-1), and human (HT-29) cell cultures. We optimized a six-phage cocktail by selecting the five most effective myoviruses and a siphovirus that have optimal lysis on prevalent serovars. We observed ∼20% of 7 log10 PFU/well phage and 3-6 log10 CFU bacterial adhesions, and 3-5 log10 CFU bacterial invasion per 2 cm2 of the cultured cells at 2 h post-treatment. The invasive bacteria when plated had a variable reduced susceptibility to the phages. After phage application at an MOI of 10, the prophylaxis regimen had better efficacy at controlling bacterial growth with an up to 6 log10 CFU/well reduction as compared with the 1-2 log10 CFU/well bacterial reduction observed in the remedial and coinfection regimens. Our data support the development of these phages to control salmonellosis in chickens, pigs, and humans.