Evaluation of recombinant cathepsin L for immunodiagnosis of gnathostomiasis
2
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Supaporn Nuamtanong, สุภาพร น่วมทะนง, Paron Dekumyoy, พารณ ดีคำย้อย, Poom Adisakwattana, ภูมิ อดิศักดิ์วัฒนา (2014). Evaluation of recombinant cathepsin L for immunodiagnosis of gnathostomiasis. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63165
Title
Evaluation of recombinant cathepsin L for immunodiagnosis of gnathostomiasis
Other Contributor(s)
Abstract
Gnathostoma spinigerum infection is a serious health problem occurring in
several countries, including Thailand. The gold standard for diagnosis is
currently detection of antibody- specific 24 kDa antigens with immunoblotting.
However, this technique requires lengthy antigen preparation, and experience to interpret
correctly. In this study, recombinant antigen, cathepsin L (GsCatL), of G.spinigerum was
evaluated as an immunodiagnostic tool. Several previous studies suggested cathepsin
L as a potential antigen for the diagnosis of helminthic infections. Initially, GsCatLcDNA
was cloned into a prokaryotic expression vector and then expressed as recombinant
GsCatL (rGsCatL) in Escherichia coli. The rGsCatL expressed at the molecular size of
48 kDa was purified and evaluated with gnathostomiasis-, other helminthic infected-and
healthy sera using immunoblot and indirect ELISA. The results showed that rGsCatL
cross-reacted with all sera by immunoblot with lowsensitivity (25.7%) and specificity
(41%). The indirect ELISA also exhibited the same result as immunoblotting. These
results suggest that rGsCatLusing a prokaryotic expression system is not suitable for
diagnosis of gnathostomiasis. However, a eukaryotic expression system still needs to
be verified for correct conformational structure through further evaluation.
Description
Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 248.