Anatomical and molecular insights into the antennal gland of the giant freshwater prawn Macrobrachium rosenbergii
Issued Date
2024-01-01
Resource Type
ISSN
0302766X
eISSN
14320878
Scopus ID
2-s2.0-85196033893
Journal Title
Cell and Tissue Research
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cell and Tissue Research (2024)
Suggested Citation
Kruangkum T., Jaiboon K., Pakawanit P., Saetan J., Pudgerd A., Wannapaiboon S., Chotwiwatthanakun C., Cummins S.F., Sobhon P., Vanichviriyakit R. Anatomical and molecular insights into the antennal gland of the giant freshwater prawn Macrobrachium rosenbergii. Cell and Tissue Research (2024). doi:10.1007/s00441-024-03898-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98909
Title
Anatomical and molecular insights into the antennal gland of the giant freshwater prawn Macrobrachium rosenbergii
Corresponding Author(s)
Other Contributor(s)
Abstract
In this study, the complex organization of the AnG in the giant freshwater prawn Macrobrachium rosenbergii was revealed using various techniques, including conventional histology, histochemistry, scanning electron microscopy, and X-ray tomography. The results showed the diversity of cells in the AnG and the detailed organization of the labyrinth’s tubule into four radiated areas from the central to peripheral zones. The study also demonstrated the expression of some vertebrate kidney-associated homolog genes, aquaporin (AQP), solute carrier family 22 (SLC-22), nephrin, and uromodulin, in the AnG by qPCR. The result of in situ hybridization further showed the localization of SLC-22 and AQP transcript in the bladder and labyrinth’s epithelium, specifically in regions 2, 3, and 4. Additionally, the study revealed neuropeptide expressions in the AnG by qPCR and in situ hybridization, i.e., crustacean hyperglycemic hormone (CHH) and molt inhibiting hormone (MIH), implying that the AnG may have a role in hormone production. Moreover, male and female prawns exhibited different levels of AQP, SLC-22, nephrin, and CHH expressions during the premolt and intermolt stages, suggesting a crucial role relevant to the molting stages. In conclusion, this study clarified the complex structure of the AnG in M. rosenbergii and demonstrated for the first time the expression of vertebrate kidney-associated genes and the possible endocrine role of the AnG. Further investigation is needed to clarify the role of these genes, particularly during ecdysis. The implications of these findings could significantly advance our understanding of the AnG in decapod crustaceans.