Critical roles of sepsis-reshaped fecal virota in attenuating sepsis severity
2
Issued Date
2022-08-02
Resource Type
eISSN
16643224
Scopus ID
2-s2.0-85136109119
Pubmed ID
35983067
Journal Title
Frontiers in Immunology
Volume
13
Rights Holder(s)
SCOPUS
Bibliographic Citation
Frontiers in Immunology Vol.13 (2022)
Suggested Citation
Chancharoenthana W., Sutnu N., Visitchanakun P., Sawaswong V., Chitcharoen S., Payungporn S., Schuetz A., Schultz M.J., Leelahavanichkul A. Critical roles of sepsis-reshaped fecal virota in attenuating sepsis severity. Frontiers in Immunology Vol.13 (2022). doi:10.3389/fimmu.2022.940935 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84931
Title
Critical roles of sepsis-reshaped fecal virota in attenuating sepsis severity
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
Chulalongkorn University
Armed Forces Research Institute of Medical Sciences, Thailand
HJF
Walter Reed Army Institute of Research
Mahidol University
Nuffield Department of Medicine
Faculty of Medicine, Chulalongkorn University
Universiteit van Amsterdam
Chulalongkorn University
Armed Forces Research Institute of Medical Sciences, Thailand
HJF
Walter Reed Army Institute of Research
Mahidol University
Nuffield Department of Medicine
Faculty of Medicine, Chulalongkorn University
Universiteit van Amsterdam
Other Contributor(s)
Abstract
Because studies on all fecal organisms (bacteria, fungi, and viruses) in sepsis are rare and bacteriophages during sepsis might have adapted against gut bacteria with possible pathogenicity, cecal ligation and puncture (CLP; a sepsis mouse model) was evaluated. In fecal bacteriome, sepsis increased Bacteroides and Proteobacteria but decreased Firmicutes, while fecal virome demonstrated increased Podoviridae when compared with sham feces. There was no difference in the fungal microbiome (predominant Ascomycota in both sham and CLP mice) and the abundance of all organisms between sepsis and control groups. Interestingly, the transfers of feces from CLP mice worsened sepsis severity when compared with sham fecal transplantation, as evaluated by mortality, renal injury (serum creatinine and histology), liver damage (liver enzyme and histology), spleen apoptosis, serum cytokines, endotoxemia, and bacteremia. In contrast, the transfers of fecal viral particles from sepsis mice, but not from sham mice, attenuated inflammation in CLP sepsis possibly through the decrease in several fecal pathogenic bacteria (such as Proteobacteria, Gammaproteobacteria, and Prevotellaceae) as evaluated by fecal microbiome analysis. Perhaps the isolation of favorable bacteriophages in sepsis feces and increased abundance ex vivo before oral treatment in a high concentration are beneficial.