Cytotoxicity of propolis extracts obtained using dichloromethane and hexane solvent on human salivary gland tumor cell line
Issued Date
2022-09-01
Resource Type
ISSN
22310762
eISSN
22501002
Scopus ID
2-s2.0-85144784726
Journal Title
Journal of International Society of Preventive and Community Dentistry
Volume
12
Issue
5
Start Page
506
End Page
512
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of International Society of Preventive and Community Dentistry Vol.12 No.5 (2022) , 506-512
Suggested Citation
Kaewmuangmoon J., Charoonpatrapong K., Janebodin K. Cytotoxicity of propolis extracts obtained using dichloromethane and hexane solvent on human salivary gland tumor cell line. Journal of International Society of Preventive and Community Dentistry Vol.12 No.5 (2022) , 506-512. 512. doi:10.4103/jispcd.JISPCD_303_21 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84431
Title
Cytotoxicity of propolis extracts obtained using dichloromethane and hexane solvent on human salivary gland tumor cell line
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Aim: This in vitro study aimed to investigate the effect of propolis extracts from two different solvents on human submandibular salivary gland (HSG) tumor cell line. Materials and Methods: Propolis was extracted by dichloromethane (DCM) and hexane (HEX). Crude extracts were prepared from 6.25 to 200 μg/mL in Dulbecco's modified eagle medium without serum. Flavonoid and total phenolic contents of crude extracts were measured using a modified colorimetric method. The cytotoxicity was evaluated by 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl-tetrazolium (MTT) assay and lactate dehydrogenase (LDH) release assay. The statistics were analyzed by independent sample t-test. Results: Propolis extracts obtained using DCM and HEX exhibited comparable % yield (38.58 and 38.25) and physical characteristics and different amounts of flavonoid (0.439 ± 0.02 and 0.250 ± 0.01 mg catechin/g sample) and total phenolic compounds (3.759 ± 0.03 and 1.618 ± 0.03 mg gallic acid equivalents/g sample). The DCM group at 25, 50, 100, and 200 μg/mL as well as the HEX group at 50, 100, and 200 μg/mL significantly displayed a decrease in % cell viability and an increase in % cytotoxicity, compared with the untreated control group (P 0.05). The DCM group showed the half-maximal inhibitory concentration (IC50) of MTT (42.93 ± 2.70) and LDH (34.94 ± 0.22). The HEX group showed the IC50 of MTT (61.30 ± 5.39) and LDH (42.32 ± 1.00). Propolis extracts obtained using both DCM and HEX are effective to inhibit HSG viability. Conclusion: Regarding to the cell morphological observation, MTT and LDH assays, propolis extracts obtained using DCM and HEX exhibited the cytotoxic effect on HSG tumor cell line. Based on our knowledge, this research demonstrates the first preliminary result suggesting propolis as a natural product of choice for salivary gland cancer prevention and therapy.