Protective role of N-Trans-Feruloyltyramine in hydrogen peroxide-induced cell death / Ei Ei Phyo Myint
Issued Date
2015
Copyright Date
2015
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xiv, 51 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Medical Biochemistry and Molecular Biology))--Mahidol University, 2015
Suggested Citation
Myint, Ei Ei Phyo, 1985- Protective role of N-Trans-Feruloyltyramine in hydrogen peroxide-induced cell death / Ei Ei Phyo Myint. Thesis (M.Sc. (Medical Biochemistry and Molecular Biology))--Mahidol University, 2015. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/94021
Title
Protective role of N-Trans-Feruloyltyramine in hydrogen peroxide-induced cell death / Ei Ei Phyo Myint
Author(s)
Abstract
Reactive oxygen species (ROS) are constantly produced by mitochondrial respiration under physiological conditions. Cells use the antioxidant enzyme system to scavenge ROS and prevent cellular damage and apoptosis. In many disease conditions such as Alzheimer's disease and Parkinson's disease, overproduction of ROS overwhelms cellular antioxidant capacity, and oxidative stress plays a pivotal role in the pathophysiology of those diseases. N-trans-feruloyltyramine (NTF), a plantderived chemical, purified from a local medicinal tree called Polyalthia suberosa, possesses an antioxidant property that could decrease ROS. A recent study has concluded that NTF can effectively protect the auto-oxidation of linoleic acid. This study, therefore, aimed to evaluate the antioxidant effects of NTF in an in vitro cell model (SK-N-SH cells), induced with H₂O₂, and whether the reduction of the ROS level can lead to cytoprotection in our model. We investigated the protective effect of NTF by assessing cell viability using MTT assay, ROS level, and cell morphology. Apoptotic cell death was demonstrated by the level of the pro-apoptotic protein Bax, activated caspase-3, and the activity of caspase-3. Challenging SK-N-SH cells with 150μM of H₂O₂ increased the ROS level, induced cytotoxic changes, and decreased cell viability whereas preincubation with various concentrations of NTF in our model significantly reduced cellular ROS, attenuated cytotoxic cell death, level of Bax, and activated caspase-3. H₂O₂-induced increase in caspase-3 activity was also preventable by NTF pretreatment. According to these findings, NTF can significantly reduce cellular ROS and protect against H₂O₂ -mediated cytotoxicity and cell death in SK-NSH cells.
Description
Medical Biochemistry and Molecular Biology (Mahidol University 2015)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Medicine Siriraj Hospital
Degree Discipline
Medical Biochemistry and Molecular Biology
Degree Grantor(s)
Mahidol University