Taylor dispersion analysis using capacitively coupled contactless conductivity detector
Issued Date
2024-05-15
Resource Type
ISSN
00399140
Scopus ID
2-s2.0-85185833041
Journal Title
Talanta
Volume
272
Rights Holder(s)
SCOPUS
Bibliographic Citation
Talanta Vol.272 (2024)
Suggested Citation
Somnin C., Chamieh J., Saetear P., Cottet H. Taylor dispersion analysis using capacitively coupled contactless conductivity detector. Talanta Vol.272 (2024). doi:10.1016/j.talanta.2024.125815 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97438
Title
Taylor dispersion analysis using capacitively coupled contactless conductivity detector
Author(s)
Corresponding Author(s)
Other Contributor(s)
Abstract
Taylor dispersion analysis (TDA) is a simple and absolute method to determine the hydrodynamic radius of solutes that respond to UV or fluorescence detections. To broaden the application range of TDA, it is necessary to develop new detection modes. This study aims to study capacitively coupled contactless conductivity detector (C4D) for the analysis of charged macromolecules. The detection sensitivities and hydrodynamic radii were compared for a C4D detector and a UV detector on positively or negatively charged polymers responding both to UV and C4D (poly-L-lysine and poly(acrylamide-co-2-acrylamido-1-methyl-propanesulfonate). The influence of the composition of the background electrolyte on the detection sensitivity has been studied and optimized for C4D detection. The influence of the molar mass and of the polymer chemical charge density on the C4D and UV sensitivities of detection have been investigated based on well-characterized copolymers samples of different molar masses and charge densities. The advantages and disadvantages compared to UV detection, as well as the range of applicability of C4D detection in TDA were identified. C4D detection can be an alternative method for sizing charged polymers of reasonable molar mass (typically below 105 g mol−1) that do not absorb in UV. A decline in the sensitivity of detection in C4D was observed for higher molar masses.